| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| Myelin Basic Protein (D8X4Q) XP® Rabbit mAb | 78896 | 20 µl | 12-18 kDa | Rabbit IgG |
| CNPase (D83E10) XP® Rabbit mAb | 5664 | 20 µl | 47 kDa | Rabbit IgG |
| PLP1 (E9V1N) Rabbit mAb | 28702 | 20 µl | 20-30 kDa | Rabbit IgG |
| MAG (D4G3) XP® Rabbit mAb | 9043 | 20 µl | 100 kDa | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat |
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Description
The Oligodendrocyte Marker Antibody Sampler Kit provides an economical means of detecting proteins identified as oligodendrocyte markers by immunofluorescence and western blot.
Storage
Background
Oligodendrocytes are the myelinating glial cells of the central nervous system (CNS) (1). Myelin basic protein (MBP) is an abundant CNS myelin membrane protein that plays an important role in nerve myelination. MBP helps to adhere the cytoplasmic leaflets of adjacent oligodendrocyte membranes to one another (2). CNPase (2', 3'-cyclic nucleotide 3'-phosphodiesterase) is an enzyme highly expressed in oligodendrocytes and accounts for roughly 4% of the total myelin protein in the CNS (3). CNPase binds to tubulin heterodimers and plays a role in tubulin polymerization, and oligodendrocyte process outgrowth (4). Myelin proteolipid protein (PLP1) corresponds to the majority of myelin proteins in the CNS, providing support to axons and modulating axonal growth (5). Myelin-associated glycoprotein (MAG) is localized in oligodendroglial membranes of myelin sheaths where it plays a role in interaction between axons and glia, and has been shown to promote axonal protective effects (6,7).
- Bradl, M. and Lassmann, H. (2010) Acta Neuropathol 119, 37-53.
- Harauz, G. and Boggs, J.M. (2013) J Neurochem 125, 334-61.
- Kozlov, G. et al. (2003) J Biol Chem 278, 46021-8.
- Lee, J. et al. (2005) J Cell Biol 170, 661-73.
- Thomson, C.E. et al. (2005) Dev Neurosci 27, 27-36.
- Quarles, R.H. (2007) J Neurochem 100, 1431-48.
- Nguyen, T. et al. (2009) J Neurosci 29, 630-7.
Background References
Trademarks and Patents
Limited Uses
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