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30728
Oligodendrocyte Marker Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Oligodendrocyte Marker Antibody Sampler Kit #30728

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Confocal immunofluorescent analysis of fixed frozen mouse subiculum from wild-type (left) or an amyloid mouse model of Alzheimer's disease (right) using PLP1 (E9V1N) Rabbit mAb (green). After blocking free secondary antibody binding sites with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, the tissue was then labeled using β-Amyloid (D54D2) XP® Rabbit mAb (Alexa Fluor® 594 Conjugate) #35363 (red) and ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Confocal immunofluorescent analysis of fixed frozen mouse hippocampus from wild-type (left) or an amyloid mouse model of Alzheimer's disease (right) using PLP1 (E9V1N) Rabbit mAb (green). After blocking free secondary antibody binding sites with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, the tissue was then labeled using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) (red) and ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Western blot analysis of extracts from various tissues using PLP1 (E9V1N) Rabbit mAb (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). The upper band corresponds to the full-length PLP1 while the lower band is an alternative splice from PLP1 known as DM20.
Western blot analysis of extracts from various brain tissues using CNPase (D83E10) XP® Rabbit mAb.
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
Western blot analysis of extracts from mouse brain and C2C12 cells using Myelin Basic Protein (D8X4Q) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western blot analysis of extracts from mouse brain, rat brain, and human cerebellum using MAG (D4G3) XP® Rabbit mAb.
Confocal immunofluorescent analysis of mouse cerebellum (left) and striatum (right) using PLP1 (E9V1N) Rabbit mAb (green) and GFAP (GA5) Mouse mAb #3670 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Confocal immunofluorescent analysis of rat cerebellum using CNPase (D83E10) XP® Rabbit mAb (green) and α/β-Synuclein (Syn205) Mouse mAb #2644 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Immunohistochemical analysis of a paraffin-embedded human Alzheimer's brain using Myelin Basic Protein (D8X4Q) XP® Rabbit mAb.
Confocal immunofluorescent analysis of rat cerebellum using MAG (D4G3) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Immunohistochemical analysis of a paraffin-embedded mouse brain using Myelin Basic Protein (D8X4Q) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Confocal immunofluorescent analysis of rat brain using Myelin Basic Protein (D8X4Q) XP® Rabbit mAb (green) and Synaptophysin (7H12) Mouse mAb (IF Formulated) #9020 (red). Blue pseudocolor = DRAQ5®#4084 (fluorescent DNA dye).
Confocal immunofluorescent analysis of mouse primary neurons and neuroglia using Myelin Basic Protein (D8X4Q) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Inquiry Info.# 30728

Product Description

The Oligodendrocyte Marker Antibody Sampler Kit provides an economical means of detecting proteins identified as oligodendrocyte markers by immunofluorescence and western blot.

Specificity / Sensitivity

Each antibody in the Oligodendrocyte Marker Antibody Sampler Kit detects endogenous levels of its target protein.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Ala185 of human myelin basic protein, Val81 of human CNPase protein, Arg605 of human MAG protein, and the carboxy terminus of human PLP1 protein.

Background

Oligodendrocytes are the myelinating glial cells of the central nervous system (CNS) (1). Myelin basic protein (MBP) is an abundant CNS myelin membrane protein that plays an important role in nerve myelination. MBP helps to adhere the cytoplasmic leaflets of adjacent oligodendrocyte membranes to one another (2). CNPase (2', 3'-cyclic nucleotide 3'-phosphodiesterase) is an enzyme highly expressed in oligodendrocytes and accounts for roughly 4% of the total myelin protein in the CNS (3). CNPase binds to tubulin heterodimers and plays a role in tubulin polymerization, and oligodendrocyte process outgrowth (4). Myelin proteolipid protein (PLP1) corresponds to the majority of myelin proteins in the CNS, providing support to axons and modulating axonal growth (5). Myelin-associated glycoprotein (MAG) is localized in oligodendroglial membranes of myelin sheaths where it plays a role in interaction between axons and glia, and has been shown to promote axonal protective effects (6,7).

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not for Use in Diagnostic Procedures.
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