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30728
Oligodendrocyte Marker Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Oligodendrocyte Marker Antibody Sampler Kit #30728

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Oligodendrocyte Marker Antibody Sampler Kit: Image 1

Confocal immunofluorescent analysis of rat brain using Myelin Basic Protein (D8X4Q) XP® Rabbit mAb (green) and Synaptophysin (7H12) Mouse mAb (IF Formulated) #9020 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Oligodendrocyte Marker Antibody Sampler Kit: Image 2

Confocal immunofluorescent analysis of rat cerebellum using CNPase (D83E10) XP® Rabbit mAb (green) and α/β-Synuclein (Syn205) Mouse mAb #2644 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Oligodendrocyte Marker Antibody Sampler Kit: Image 3

Confocal immunofluorescent analysis of mouse cerebellum (left) and striatum (right) using PLP1 (E9V1N) Rabbit mAb (green) and GFAP (GA5) Mouse mAb #3670 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).

Oligodendrocyte Marker Antibody Sampler Kit: Image 4

Confocal immunofluorescent analysis of rat cerebellum using MAG (D4G3) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Oligodendrocyte Marker Antibody Sampler Kit: Image 5

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Oligodendrocyte Marker Antibody Sampler Kit: Image 6

Confocal immunofluorescent analysis of mouse primary neurons and neuroglia using Myelin Basic Protein (D8X4Q) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Oligodendrocyte Marker Antibody Sampler Kit: Image 7

Western blot analysis of extracts from various brain tissues using CNPase (D83E10) XP® Rabbit mAb.

Oligodendrocyte Marker Antibody Sampler Kit: Image 8

Western blot analysis of extracts from various tissues using PLP1 (E9V1N) Rabbit mAb (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). The upper band corresponds to the full-length PLP1 while the lower band is an alternative splice from PLP1 known as DM20.

Oligodendrocyte Marker Antibody Sampler Kit: Image 9

Western blot analysis of extracts from mouse brain, rat brain, and human cerebellum using MAG (D4G3) XP® Rabbit mAb.

Oligodendrocyte Marker Antibody Sampler Kit: Image 10

Immunohistochemical analysis of a paraffin-embedded human Alzheimer's brain using Myelin Basic Protein (D8X4Q) XP® Rabbit mAb.

Oligodendrocyte Marker Antibody Sampler Kit: Image 11

Immunohistochemical analysis of a paraffin-embedded mouse brain using Myelin Basic Protein (D8X4Q) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

Oligodendrocyte Marker Antibody Sampler Kit: Image 12

Western blot analysis of extracts from mouse brain and C2C12 cells using Myelin Basic Protein (D8X4Q) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

To Purchase # 30728T
Product # Size Price
30728T
1 Kit  (4 x 20 µl) $ 327

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Myelin Basic Protein (D8X4Q) XP® Rabbit mAb 78896 20 µl
  • WB
  • IHC
  • IF
H M R 12-18 Rabbit IgG
CNPase (D83E10) XP® Rabbit mAb 5664 20 µl
  • WB
  • IP
  • IF
H M R 47 Rabbit IgG
PLP1 (E9V1N) Rabbit mAb 28702 20 µl
  • WB
  • IF
H M R 20-30 Rabbit IgG
MAG (D4G3) XP® Rabbit mAb 9043 20 µl
  • WB
  • IP
  • IF
H M R 100 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

Product Description

The Oligodendrocyte Marker Antibody Sampler Kit provides an economical means of detecting proteins identified as oligodendrocyte markers by immunofluorescence and western blot.

Specificity / Sensitivity

Each antibody in the Oligodendrocyte Marker Antibody Sampler Kit detects endogenous levels of its target protein.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Ala185 of human myelin basic protein, Val81 of human CNPase protein, Arg605 of human MAG protein, and the carboxy terminus of human PLP1 protein.

Background

Oligodendrocytes are the myelinating glial cells of the central nervous system (CNS) (1). Myelin basic protein (MBP) is an abundant CNS myelin membrane protein that plays an important role in nerve myelination. MBP helps to adhere the cytoplasmic leaflets of adjacent oligodendrocyte membranes to one another (2). CNPase (2', 3'-cyclic nucleotide 3'-phosphodiesterase) is an enzyme highly expressed in oligodendrocytes and accounts for roughly 4% of the total myelin protein in the CNS (3). CNPase binds to tubulin heterodimers and plays a role in tubulin polymerization, and oligodendrocyte process outgrowth (4). Myelin proteolipid protein (PLP1) corresponds to the majority of myelin proteins in the CNS, providing support to axons and modulating axonal growth (5). Myelin-associated glycoprotein (MAG) is localized in oligodendroglial membranes of myelin sheaths where it plays a role in interaction between axons and glia, and has been shown to promote axonal protective effects (6,7).

  1. Bradl, M. and Lassmann, H. (2010) Acta Neuropathol 119, 37-53.
  2. Harauz, G. and Boggs, J.M. (2013) J Neurochem 125, 334-61.
  3. Kozlov, G. et al. (2003) J Biol Chem 278, 46021-8.
  4. Lee, J. et al. (2005) J Cell Biol 170, 661-73.
  5. Thomson, C.E. et al. Dev Neurosci 27, 27-36.
  6. Quarles, R.H. (2007) J Neurochem 100, 1431-48.
  7. Nguyen, T. et al. (2009) J Neurosci 29, 630-7.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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