Revision 1

#38289

Store at -20C

Oligodendrocyte Marker Antibody Sampler Kit

1 Kit

(8 x 20 microliters)

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Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Product Includes Product # Quantity Mol. Wt Isotype/Source
Olig2 (E6G6Q) Rabbit Monoclonal Antibody6591520 µl35 kDaRabbit IgG
Myelin Basic Protein (D8X4Q) Rabbit Monoclonal Antibody7889620 µl12-18 kDaRabbit IgG
CNPase (D83E10) Rabbit Monoclonal Antibody566420 µl47 kDaRabbit IgG
PLP1 (E9V1N) Rabbit Monoclonal Antibody2870220 µl20-30 kDaRabbit IgG
MAG (D4G3) Rabbit Monoclonal Antibody904320 µl100 kDaRabbit IgG
MOG (E5K6T) Rabbit Monoclonal Antibody9645720 µl46, 35, 28, 23 kDaRabbit IgG
NG2/CSPG4 (E3B3G) Rabbit Monoclonal Antibody4391620 µl250, 450 kDaRabbit IgG
Sox10 (E6B6I) Rabbit Monoclonal Antibody6966120 µl62 kDaRabbit IgG
Anti-rabbit IgG, HRP-linked Antibody7074100 µlGoat

Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.

Description

The Oligodendrocyte Marker Antibody Sampler Kit provides an economical means of detecting proteins identified as oligodendrocyte markers by immunofluorescence and western blot. This kit includes enough primary antibodies to perform at least twenty IF tests or two western blot experiments per primary antibody.

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibodies.

Background

Oligodendrocytes are the myelinating glial cells of the central nervous system (CNS) (1). Myelin basic protein (MBP) is an abundant CNS myelin membrane protein that plays an important role in nerve myelination. MBP helps to adhere the cytoplasmic leaflets of adjacent oligodendrocyte membranes to one another (2). CNPase (2', 3'-cyclic nucleotide 3'-phosphodiesterase) is an enzyme highly expressed in oligodendrocytes and accounts for roughly 4% of the total myelin protein in the CNS (3). CNPase binds to tubulin heterodimers and plays a role in tubulin polymerization, and oligodendrocyte process outgrowth (4). Myelin proteolipid protein (PLP1) corresponds to the majority of myelin proteins in the CNS, providing support to axons and modulating axonal growth (5). Myelin-associated glycoprotein (MAG) is localized in oligodendroglial membranes of myelin sheaths where it plays a role in interaction between axons and glia, and has been shown to promote axonal protective effects (6,7). Chondroitin sulfate proteoglycan 4 (CSP4, NG2) is a type I membrane glycoprotein expressed by oligodendroglial precursor cells (OPCs) that plays a role in cell attachment and migration (8). SRY-box 10 (Sox10) is a high-mobility group transcription factor expressed throughout oligodendrocyte development required for myelin gene expression (9,10). Myelin-oligodendrocyte glycoprotein (MOG) is a type I membrane bound glycoprotein of the immunoglobulin superfamily that is enriched in the outer lamella of the myelin sheath (11). Olig2 is a basic helix–loop–helix (bHLH) transcription factor necessary for oligodendrocyte and motor neuron differentiation and development in the CNS (12,13). Olig2 has been shown to be a useful marker for primary and mature oligodendrocytes and is universally expressed in diffuse gliomas (14,15).

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Western blot analysis of extracts from various tissues using PLP1 (E9V1N) Rabbit mAb (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). The upper band corresponds to the full-length PLP1 while the lower band is an alternative splice from PLP1 known as DM20.
undefined Image 1: Oligodendrocyte Marker Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human Alzheimer's brain using PLP1 (E9V1N) Rabbit mAb.
undefined Image 2: Oligodendrocyte Marker Antibody Sampler Kit
Western blot analysis of extracts from various cell lines using NG2/CSPG4 (E3B3G) XP® Rabbit mAb (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). As expected, NG2/CSPG4 protein is not expressed in MCF7 cells.
undefined Image 3: Oligodendrocyte Marker Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Western blot analysis of extracts from various brain tissues using CNPase (D83E10) XP® Rabbit mAb.
undefined Image 4: Oligodendrocyte Marker Antibody Sampler Kit
Western blot analysis of extracts from mouse, rat, and human brain tissue using Olig2 (E6G6Q) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
undefined Image 5: Oligodendrocyte Marker Antibody Sampler Kit
Western blot analysis of extracts from SK-MEL-28 and HeLa cells using Sox10 (E6B6I) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). As expected, HeLa is low or negative for Sox10 expression.
undefined Image 6: Oligodendrocyte Marker Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

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After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
undefined Image 7: Oligodendrocyte Marker Antibody Sampler Kit
Western blot analysis of extracts from mouse brain and C2C12 cells using Myelin Basic Protein (D8X4Q) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
undefined Image 8: Oligodendrocyte Marker Antibody Sampler Kit
Western blot analysis of extracts from mouse brain, rat brain, and human cerebellum using MAG (D4G3) XP® Rabbit mAb.
undefined Image 9: Oligodendrocyte Marker Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Confocal immunofluorescent analysis of fixed frozen mouse cerebellum using MAG (D4G3) Rabbit Monoclonal Antibody (green) and ProLong Gold Antifade Reagent with DAPI #8961 (blue).
undefined Image 10: Oligodendrocyte Marker Antibody Sampler Kit
Western blot analysis of extracts from various tissues using MOG (E5K6T) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
undefined Image 11: Oligodendrocyte Marker Antibody Sampler Kit
Confocal immunofluorescent analysis of mouse cerebellum (left) and striatum (right) using PLP1 (E9V1N) Rabbit mAb (green) and GFAP (GA5) Mouse mAb #3670 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
undefined Image 12: Oligodendrocyte Marker Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human glioma using PLP1 (E9V1N) Rabbit mAb.
undefined Image 13: Oligodendrocyte Marker Antibody Sampler Kit
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human NG2/CSPG4 protein (hNG2/CSPG4-Myc/DDK; +), using NG2/CSPG4 (E3B3G) XP® Rabbit mAb (upper), DYKDDDDK Tag Antibody #2368 (middle), and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).
undefined Image 14: Oligodendrocyte Marker Antibody Sampler Kit
Simple Western™ analysis of lysates (0.1 mg/mL) from Mouse Brain cells using CNPase (D83E10) XP® Rabbit mAb #5664. The virtual lane view (left) shows the target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.
undefined Image 15: Oligodendrocyte Marker Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Simple Western™ analysis of lysates (0.75 mg/mL) from Mouse Brain tissue using Olig2 (E6G6Q) XP® Rabbit mAb #65915. The virtual lane view (left) shows the target band (as indicated) at 1:50 and 1:250 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:50 (blue line) and 1:250 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.
undefined Image 16: Oligodendrocyte Marker Antibody Sampler Kit
Immunoprecipitation of Sox10 from SK-MEL-5 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Sox10 (E6B6I) XP® Rabbit mAb. Western blot analysis was performed usingSox10 (E6B6I) XP® Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as the secondary antibody.
undefined Image 17: Oligodendrocyte Marker Antibody Sampler Kit
Immunohistochemical analysis of a paraffin-embedded human Alzheimer's brain using Myelin Basic Protein (D8X4Q) XP® Rabbit mAb.
undefined Image 18: Oligodendrocyte Marker Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Simple Western™ analysis of lysates (0.1 mg/mL) from Mouse Brain tissue using MAG (D4G3) XP® Rabbit mAb #9043. The virtual lane view (left) shows the target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 66-440 kDa separation module.
undefined Image 19: Oligodendrocyte Marker Antibody Sampler Kit
Simple Western™ analysis of lysates (0.1 mg/mL) from Mouse Brain tissue using MAG (D4G3) XP® Rabbit mAb #9043. The virtual lane view (left) shows the target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 66-440 kDa separation module.
undefined Image 20: Oligodendrocyte Marker Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human cerebellum using MOG (E5K6T) XP® Rabbit mAb.
undefined Image 21: Oligodendrocyte Marker Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human glioblastoma using PLP1 (E9V1N) Rabbit mAb.
undefined Image 22: Oligodendrocyte Marker Antibody Sampler Kit
Western blot analysis of extracts from HeLa cells, either wild-type (+/+) or CSPG4 knockout (-/-), using NG2/CSPG4 (E3B3G) XP® Rabbit mAb (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).
undefined Image 23: Oligodendrocyte Marker Antibody Sampler Kit
Confocal immunofluorescent analysis of rat cerebellum using CNPase (D83E10) XP® Rabbit mAb (green) and α/β-Synuclein (Syn205) Mouse mAb #2644 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
undefined Image 24: Oligodendrocyte Marker Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

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Immunoprecipitation of Olig2 protein from mouse brain tissue extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Olig2 (E6G6Q) XP® Rabbit mAb. Western blot analysis was performed using Olig2 (E6G6Q) XP® Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody.
undefined Image 25: Oligodendrocyte Marker Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using Sox10 (E6B6I) XP® Rabbit mAb performed on the Leica® BOND™ Rx.
undefined Image 26: Oligodendrocyte Marker Antibody Sampler Kit
Immunohistochemical analysis of a paraffin-embedded mouse brain using Myelin Basic Protein (D8X4Q) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
undefined Image 27: Oligodendrocyte Marker Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded mouse brain using MOG (E5K6T) XP® Rabbit mAb.
undefined Image 28: Oligodendrocyte Marker Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded normal human brain using PLP1 (E9V1N) Rabbit mAb.
undefined Image 29: Oligodendrocyte Marker Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using NG2/CSPG4 (E3B3G) XP® Rabbit mAb.
undefined Image 30: Oligodendrocyte Marker Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

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Immunohistochemical analysis of paraffin-embedded normal human brain using Olig2 (E6G6Q) XP® Rabbit mAb.
undefined Image 31: Oligodendrocyte Marker Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human ductal breast carcinoma using Sox10 (E6B6I) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
undefined Image 32: Oligodendrocyte Marker Antibody Sampler Kit
Confocal immunofluorescent analysis of rat brain using Myelin Basic Protein (D8X4Q) XP® Rabbit mAb (green) and Synaptophysin (7H12) Mouse mAb (IF Formulated) #9020 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
undefined Image 33: Oligodendrocyte Marker Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

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Immunohistochemical analysis of paraffin-embedded mouse cerebellum (left, positive) or colon (right, negative) using MOG (E5K6T) XP® Rabbit mAb. Note the lack of staining of the myenteric plexus ganglion cells in the mouse colon.
undefined Image 34: Oligodendrocyte Marker Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded mouse cerebellum (left, positive), small intestine (middle, negative), or LL/2 syngeneic tumor (right, negative) using PLP1 (E9V1N) Rabbit mAb.
undefined Image 35: Oligodendrocyte Marker Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human oral squamous cell carcinoma using NG2/CSPG4 (E3B3G) XP® Rabbit mAb.
undefined Image 36: Oligodendrocyte Marker Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

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Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Olig2 (E6G6Q) XP® Rabbit mAb.
undefined Image 37: Oligodendrocyte Marker Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human squamous carcinoma of the tongue using Sox10 (E6B6I) XP® Rabbit mAb.
undefined Image 38: Oligodendrocyte Marker Antibody Sampler Kit
Confocal immunofluorescent analysis of mouse primary neurons and neuroglia using Myelin Basic Protein (D8X4Q) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
undefined Image 39: Oligodendrocyte Marker Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded rat brain using MOG (E5K6T) XP® Rabbit mAb.
undefined Image 40: Oligodendrocyte Marker Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded normal human brain using PLP1 (E9V1N) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
undefined Image 41: Oligodendrocyte Marker Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using NG2/CSPG4 (E3B3G) XP® Rabbit mAb.
undefined Image 42: Oligodendrocyte Marker Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

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Immunohistochemical analysis of paraffin-embedded human appendix using Olig2 (E6G6Q) XP® Rabbit mAb.
undefined Image 43: Oligodendrocyte Marker Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human melanoma using Sox10 (E6B6I) XP® Rabbit mAb.
undefined Image 44: Oligodendrocyte Marker Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human gastrointestinal stromal tumor (left), normal liver (middle), or normal lung (right), using NG2/CSPG4 (E3B3G) XP® Rabbit mAb (top) or NG2/CSPG4 Rabbit mAb (bottom). These two antibodies detect independent, unique epitopes on human NG2/CSPG4. The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining.
undefined Image 45: Oligodendrocyte Marker Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

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Immunohistochemical analysis of paraffin-embedded mouse brain, hippocampus (left), cerebellum (middle), and a nerve fiber tract in the striatum (right) using Olig2 (E6G6Q) XP® Rabbit mAb.
undefined Image 46: Oligodendrocyte Marker Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded SK-MEL-5 cell pellet (left, positive) or HeLa cell pellet (right, negative) using Sox10 (E6B6I) XP® Rabbit mAb.
undefined Image 47: Oligodendrocyte Marker Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded normal human kidney using NG2/CSPG4 (E3B3G) XP® Rabbit mAb.
undefined Image 48: Oligodendrocyte Marker Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

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Immunohistochemical analysis of paraffin-embedded GL261 syngeneic tumor (left, positive) and Renca syngeneic tumor (right, negative) using Olig2 (E6G6Q) XP® Rabbit mAb.
undefined Image 49: Oligodendrocyte Marker Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma using Sox10 (E6B6I) XP® Rabbit mAb.
undefined Image 50: Oligodendrocyte Marker Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human B-cell non-Hodgkin's lymphoma using NG2/CSPG4 (E3B3G) XP® Rabbit mAb.
undefined Image 51: Oligodendrocyte Marker Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

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Immunohistochemical analysis of paraffin-embedded GL261 syngeneic tumor using Olig2 (E6G6Q) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
undefined Image 52: Oligodendrocyte Marker Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded A-375 cell pellet (left, positive) or MCF7 cell pellet (right, negative) using NG2/CSPG4 (E3B3G) XP® Rabbit mAb.
undefined Image 53: Oligodendrocyte Marker Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human oligodendroglioma using Olig2 (E6G6Q) XP® Rabbit mAb.
undefined Image 54: Oligodendrocyte Marker Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

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Confocal immunofluorescent analysis of A-375 cells (left, positive) or MCF7 cells (right, negative) using NG2/CSPG4 (E3B3G) XP® Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Sections were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
undefined Image 55: Oligodendrocyte Marker Antibody Sampler Kit
Confocal immunofluorescent analysis of fixed frozen mouse cerebellum, labeled with Olig2 (E6G6Q) XP® Rabbit mAb (left, red) and co-labeled with GFAP (GA5) Mouse mAb (Alexa Fluor® 488 Conjugate) #3655 (right, green) and DAPI #4083 (right, blue).
undefined Image 56: Oligodendrocyte Marker Antibody Sampler Kit
Confocal immunofluorescent analysis of fixed frozen postnatal day five (P5) mouse hippocampus, labeled with Olig2 (E6G6Q) XP® Rabbit mAb (left, red) and co-labeled with GFAP (GA5) Mouse mAb (Alexa Fluor® 488 Conjugate) #3655 (right, green) and DAPI #4083 (right, blue).
undefined Image 57: Oligodendrocyte Marker Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

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Confocal immunofluorescent analysis of fixed frozen mouse subiculum from wild-type (left) or an amyloid mouse model of Alzheimer's disease (right) using PLP1 (E9V1N) Rabbit mAb (green). After blocking free secondary antibody binding sites with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, the tissue was then labeled using β-Amyloid (D54D2) XP® Rabbit mAb (Alexa Fluor® 594 Conjugate) #35363 (red) and ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
undefined Image 58: Oligodendrocyte Marker Antibody Sampler Kit
Confocal immunofluorescent analysis of fixed frozen mouse hippocampus from wild-type (left) or an amyloid mouse model of Alzheimer's disease (right) using PLP1 (E9V1N) Rabbit mAb (green). After blocking free secondary antibody binding sites with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, the tissue was then labeled using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) (red) and ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
undefined Image 59: Oligodendrocyte Marker Antibody Sampler Kit
Simple Western™ analysis of lysates (0.05 mg/mL) from mouse brain tissue using PLP1 (E9V1N) Rabbit mAb #28702. The virtual lane view (left) shows the target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.
undefined Image 60: Oligodendrocyte Marker Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

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Cyclic multiplex immunofluorescent analysis of fixed frozen hippocampus in a normal mouse (left) or an amyloid mouse model of Alzheimer's Disease (right) using PLP1 (E9V1N) Rabbit mAb (red). After blocking free secondary antibody binding sites with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, the tissues were then labeled using Myelin Basic Protein (D8X4Q) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #30103 (green) and β-Amyloid (D54D2) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #51374 (cyan). Sections were mounted in ProLong Gold Antifade Reagent with DAPI #8961 (blue) and then imaged. After imaging, sections were processed in Western Blot Stripping Buffer (5X) #91925 to remove bound primary and secondary antibody. Then sections were labeled with GFAP (E4L7M) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #94242 (gray) and Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #78060 (magenta). Sections were mounted in ProLong Gold Antifade Reagent with DAPI #8961 (blue) and then imaged a second time.
undefined Image 61: Oligodendrocyte Marker Antibody Sampler Kit
Cyclic multiplex immunofluorescent analysis of fixed frozen cortex in a normal mouse (left) or an amyloid mouse model of Alzheimer's Disease (right) using PLP1 (E9V1N) Rabbit mAb #28702 (red). After blocking free secondary antibody binding sites with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, the tissues were then labeled using Myelin Basic Protein (D8X4Q) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #30103 (green) and β-Amyloid (D54D2) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #51374 (cyan). Sections were mounted in ProLong Gold Antifade Reagent with DAPI #8961 (blue) and then imaged. After imaging, sections were processed in Western Blot Stripping Buffer (5X) #91925 to remove bound primary and secondary antibody. Then sections were labeled with GFAP (E4L7M) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #94242 (gray) and Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #78060 (magenta). Sections were mounted in ProLong Gold Antifade Reagent with DAPI #8961 (blue) and then imaged a second time.
undefined Image 62: Oligodendrocyte Marker Antibody Sampler Kit
Simple WesternTM analysis of lysates (1.0 mg/mL) from A172 using NG2/CSPG4 (E3B3G) XP® Rabbit mAb #43916.  The virtual lane view (left) shows a single target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody.  The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody.  This experiment was performed under reducing conditions on the JessTM Simple Western instrument from ProteinSimple, a BioTechne brand, using the 66-440 kDa separation module.
undefined Image 63: Oligodendrocyte Marker Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

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Confocal immunofluorescent analysis of SK-MEL-5 cells (left, positive) and SK-OV-3 cells (right, negative) using Olig2 (E6G6Q) XP® Rabbit mAb (green), DyLight 554 Phalloidin #13054 (red), and DAPI #4083 (blue).
undefined Image 64: Oligodendrocyte Marker Antibody Sampler Kit
Simple Western™ analysis of lysates (0.1 mg/mL) from mouse brain using Myelin Basic Protein (D8X4Q) XP® Rabbit mAb #78896. The virtual lane view (left) shows the target band (as indicated) at 1:50 and 1:250 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:50 (blue line) and 1:250 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 2-40 kDa separation module.
undefined Image 65: Oligodendrocyte Marker Antibody Sampler Kit
Simple Western™ analysis of lysates (0.1 mg/mL) from Mouse Brain tissue using MOG (E5K6T) XP® Rabbit mAb #96457. The virtual lane view (left) shows the target band (as indicated) at 1:50 and 1:250 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:50 (blue line) and 1:250 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.
undefined Image 66: Oligodendrocyte Marker Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.