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38289
Oligodendrocyte Marker Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit
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Oligodendrocyte Marker Antibody Sampler Kit #38289

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Oligodendrocyte Marker Antibody Sampler Kit: Image 1
Western blot analysis of extracts from various tissues using PLP1 (E9V1N) Rabbit mAb (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). The upper band corresponds to the full-length PLP1 while the lower band is an alternative splice from PLP1 known as DM20.
Oligodendrocyte Marker Antibody Sampler Kit: Image 2
Western blot analysis of extracts from various cell lines using NG2/CSPG4 (E3B3G) XP® Rabbit mAb (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). As expected, NG2/CSPG4 protein is not expressed in MCF7 cells.
Oligodendrocyte Marker Antibody Sampler Kit: Image 3
Western blot analysis of extracts from various brain tissues using CNPase (D83E10) XP® Rabbit mAb.
Oligodendrocyte Marker Antibody Sampler Kit: Image 4
Immunohistochemical analysis of paraffin-embedded human oligodendroglioma using Olig2 (E6G6Q) XP® Rabbit mAb.
Oligodendrocyte Marker Antibody Sampler Kit: Image 5
Immunoprecipitation of Olig2 protein from mouse brain tissue extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Olig2 (E6G6Q) XP® Rabbit mAb. Western blot analysis was performed using Olig2 (E6G6Q) XP® Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody.
Oligodendrocyte Marker Antibody Sampler Kit: Image 6
Western blot analysis of extracts from mouse, rat, and human brain tissue using Olig2 (E6G6Q) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Oligodendrocyte Marker Antibody Sampler Kit: Image 7
Confocal immunofluorescent analysis of fixed frozen postnatal day five (P5) mouse hippocampus, labeled with Olig2 (E6G6Q) XP® Rabbit mAb (left, red) and co-labeled with GFAP (GA5) Mouse mAb (Alexa Fluor® 488 Conjugate) #3655 (right, green) and DAPI #4083 (right, blue).
Oligodendrocyte Marker Antibody Sampler Kit: Image 8
Confocal immunofluorescent analysis of fixed frozen mouse cerebellum, labeled with Olig2 (E6G6Q) XP® Rabbit mAb (left, red) and co-labeled with GFAP (GA5) Mouse mAb (Alexa Fluor® 488 Conjugate) #3655 (right, green) and DAPI #4083 (right, blue).
Oligodendrocyte Marker Antibody Sampler Kit: Image 9
Western blot analysis of extracts from SK-MEL-28 and HeLa cells using Sox10 (E6B6I) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). As expected, HeLa is low or negative for Sox10 expression.
Oligodendrocyte Marker Antibody Sampler Kit: Image 10
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
Oligodendrocyte Marker Antibody Sampler Kit: Image 11
Western blot analysis of extracts from mouse brain and C2C12 cells using Myelin Basic Protein (D8X4Q) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Oligodendrocyte Marker Antibody Sampler Kit: Image 12
Western blot analysis of extracts from mouse brain, rat brain, and human cerebellum using MAG (D4G3) XP® Rabbit mAb.
Oligodendrocyte Marker Antibody Sampler Kit: Image 13
Western blot analysis of extracts from various tissues using MOG (E5K6T) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Oligodendrocyte Marker Antibody Sampler Kit: Image 14
Confocal immunofluorescent analysis of mouse cerebellum (left) and striatum (right) using PLP1 (E9V1N) Rabbit mAb (green) and GFAP (GA5) Mouse mAb #3670 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Oligodendrocyte Marker Antibody Sampler Kit: Image 15
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human NG2/CSPG4 protein (hNG2/CSPG4-Myc/DDK; +), using NG2/CSPG4 (E3B3G) XP® Rabbit mAb (upper), DYKDDDDK Tag Antibody #2368 (middle), and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).
Oligodendrocyte Marker Antibody Sampler Kit: Image 16
Confocal immunofluorescent analysis of rat cerebellum using CNPase (D83E10) XP® Rabbit mAb (green) and α/β-Synuclein (Syn205) Mouse mAb #2644 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Oligodendrocyte Marker Antibody Sampler Kit: Image 17
Immunohistochemical analysis of paraffin-embedded normal human brain using Olig2 (E6G6Q) XP® Rabbit mAb.
Oligodendrocyte Marker Antibody Sampler Kit: Image 18
Immunoprecipitation of Sox10 from SK-MEL-5 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Sox10 (E6B6I) XP® Rabbit mAb. Western blot analysis was performed using

Sox10 (E6B6I) XP® Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as the secondary antibody.

Oligodendrocyte Marker Antibody Sampler Kit: Image 19
Immunohistochemical analysis of a paraffin-embedded human Alzheimer's brain using Myelin Basic Protein (D8X4Q) XP® Rabbit mAb.
Oligodendrocyte Marker Antibody Sampler Kit: Image 20
Confocal immunofluorescent analysis of rat cerebellum using MAG (D4G3) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Oligodendrocyte Marker Antibody Sampler Kit: Image 21
Immunohistochemical analysis of paraffin-embedded human cerebellum using MOG (E5K6T) XP® Rabbit mAb.
Oligodendrocyte Marker Antibody Sampler Kit: Image 22
Western blot analysis of extracts from HeLa cells, either wild-type (+/+) or CSPG4 knockout (-/-), using NG2/CSPG4 (E3B3G) XP® Rabbit mAb (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).
Oligodendrocyte Marker Antibody Sampler Kit: Image 23
Immunohistochemical analysis of paraffin-embedded human appendix using Olig2 (E6G6Q) XP® Rabbit mAb.
Oligodendrocyte Marker Antibody Sampler Kit: Image 24
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using Sox10 (E6B6I) XP® Rabbit mAb performed on the Leica® BOND™ Rx.
Oligodendrocyte Marker Antibody Sampler Kit: Image 25
Immunohistochemical analysis of a paraffin-embedded mouse brain using Myelin Basic Protein (D8X4Q) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Oligodendrocyte Marker Antibody Sampler Kit: Image 26
Immunohistochemical analysis of paraffin-embedded mouse brain using MOG (E5K6T) XP® Rabbit mAb.
Oligodendrocyte Marker Antibody Sampler Kit: Image 27
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using NG2/CSPG4 (E3B3G) XP® Rabbit mAb.
Oligodendrocyte Marker Antibody Sampler Kit: Image 28
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Olig2 (E6G6Q) XP® Rabbit mAb.
Oligodendrocyte Marker Antibody Sampler Kit: Image 29
Immunohistochemical analysis of paraffin-embedded human ductal breast carcinoma using Sox10 (E6B6I) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Oligodendrocyte Marker Antibody Sampler Kit: Image 30
Confocal immunofluorescent analysis of rat brain using Myelin Basic Protein (D8X4Q) XP® Rabbit mAb (green) and Synaptophysin (7H12) Mouse mAb (IF Formulated) #9020 (red). Blue pseudocolor = DRAQ5®#4084 (fluorescent DNA dye).
Oligodendrocyte Marker Antibody Sampler Kit: Image 31
Immunohistochemical analysis of paraffin-embedded mouse cerebellum (left, positive) or colon (right, negative) using MOG (E5K6T) XP® Rabbit mAb. Note the lack of staining of the myenteric plexus ganglion cells in the mouse colon.
Oligodendrocyte Marker Antibody Sampler Kit: Image 32
Immunohistochemical analysis of paraffin-embedded human oral squamous cell carcinoma using NG2/CSPG4 (E3B3G) XP® Rabbit mAb.
Oligodendrocyte Marker Antibody Sampler Kit: Image 33
Immunohistochemical analysis of paraffin-embedded mouse brain, hippocampus (left), cerebellum (middle), and a nerve fiber tract in the striatum (right) using Olig2 (E6G6Q) XP® Rabbit mAb.
Oligodendrocyte Marker Antibody Sampler Kit: Image 34
Immunohistochemical analysis of paraffin-embedded human squamous carcinoma of the tongue using Sox10 (E6B6I) XP® Rabbit mAb.
Oligodendrocyte Marker Antibody Sampler Kit: Image 35
Confocal immunofluorescent analysis of mouse primary neurons and neuroglia using Myelin Basic Protein (D8X4Q) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Oligodendrocyte Marker Antibody Sampler Kit: Image 36
Immunohistochemical analysis of paraffin-embedded rat brain using MOG (E5K6T) XP® Rabbit mAb.
Oligodendrocyte Marker Antibody Sampler Kit: Image 37
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using NG2/CSPG4 (E3B3G) XP® Rabbit mAb.
Oligodendrocyte Marker Antibody Sampler Kit: Image 38
Immunohistochemical analysis of paraffin-embedded GL261 syngeneic tumor (left, positive) and Renca syngeneic tumor (right, negative) using Olig2 (E6G6Q) XP® Rabbit mAb.
Oligodendrocyte Marker Antibody Sampler Kit: Image 39
Immunohistochemical analysis of paraffin-embedded human melanoma using Sox10 (E6B6I) XP® Rabbit mAb.
Oligodendrocyte Marker Antibody Sampler Kit: Image 40
Immunohistochemical analysis of paraffin-embedded human gastrointestinal stromal tumor (left), normal liver (middle), or normal lung (right), using NG2/CSPG4 (E3B3G) XP® Rabbit mAb (top) or NG2/CSPG4 Rabbit mAb (bottom). These two antibodies detect independent, unique epitopes on human NG2/CSPG4. The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining.
Oligodendrocyte Marker Antibody Sampler Kit: Image 41
Immunohistochemical analysis of paraffin-embedded GL261 syngeneic tumor using Olig2 (E6G6Q) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Oligodendrocyte Marker Antibody Sampler Kit: Image 42
Immunohistochemical analysis of paraffin-embedded SK-MEL-5 cell pellet (left, positive) or HeLa cell pellet (right, negative) using Sox10 (E6B6I) XP® Rabbit mAb.
Oligodendrocyte Marker Antibody Sampler Kit: Image 43
Immunohistochemical analysis of paraffin-embedded normal human kidney using NG2/CSPG4 (E3B3G) XP® Rabbit mAb.
Oligodendrocyte Marker Antibody Sampler Kit: Image 44
Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma using Sox10 (E6B6I) XP® Rabbit mAb.
Oligodendrocyte Marker Antibody Sampler Kit: Image 45
Immunohistochemical analysis of paraffin-embedded human B-cell non-Hodgkin's lymphoma using NG2/CSPG4 (E3B3G) XP® Rabbit mAb.
Oligodendrocyte Marker Antibody Sampler Kit: Image 46
Immunohistochemical analysis of paraffin-embedded A-375 cell pellet (left, positive) or MCF7 cell pellet (right, negative) using NG2/CSPG4 (E3B3G) XP® Rabbit mAb.
Oligodendrocyte Marker Antibody Sampler Kit: Image 47
Confocal immunofluorescent analysis of A-375 cells (left, positive) or MCF7 cells (right, negative) using NG2/CSPG4 (E3B3G) XP® Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Sections were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
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To Purchase # 38289
Cat. # Size Qty. Price
38289T		 1 Kit  (8 x 20 microliters)
$ 645

Custom Formulation

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Olig2 (E6G6Q) XP® Rabbit mAb 65915 20 µl
  • WB
  • IP
  • IHC
  • IF
 H M R 35 Rabbit IgG
Myelin Basic Protein (D8X4Q) XP® Rabbit mAb 78896 20 µl
  • WB
  • IHC
  • IF
 H M R 12-18 Rabbit IgG
CNPase (D83E10) XP® Rabbit mAb 5664 20 µl
  • WB
  • IP
  • IF
 H M R 47 Rabbit IgG
PLP1 (E9V1N) Rabbit mAb 28702 20 µl
  • WB
  • IF
 H M R 20-30 Rabbit IgG
MAG (D4G3) XP® Rabbit mAb 9043 20 µl
  • WB
  • IP
  • IF
 H M R 100 Rabbit IgG
MOG (E5K6T) XP® Rabbit mAb 96457 20 µl
  • WB
  • IHC
 H M R 46, 35, 28, 23 Rabbit IgG
NG2/CSPG4 (E3B3G) XP® Rabbit mAb 43916 20 µl
  • WB
  • IHC
  • IF
 H 250, 450 Rabbit IgG
Sox10 (E6B6I) XP® Rabbit mAb 69661 20 µl
  • WB
  • IP
  • IHC
 H 62 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
 Rab Goat 

Product Description

The Oligodendrocyte Marker Antibody Sampler Kit provides an economical means of detecting proteins identified as oligodendrocyte markers by immunofluorescence and western blot. This kit includes enough primary antibodies to perform at least twenty IF tests or two western blot experiments per primary antibody.

Specificity / Sensitivity

Each antibody in the Oligodendrocyte Marker Antibody Sampler Kit detects endogenous levels of its target protein. Sox10 (E6B6I) XP® Rabbit mAb also recognizes presumptive sumoylated forms of Sox10 protein.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Ala185 of human myelin basic protein, Val81 of human CNPase protein, Arg605 of human MAG protein, Asp1410 of human NG2/CSPG4 protein, Gly446 of human Sox10 protein, recombinant protein specific to the carboxy terminus of human Olig2 protein and human PLP1 protein, and recombinant protein specific to the extracellular membrane proximal amino terminus of human MOG protein.

Background

Oligodendrocytes are the myelinating glial cells of the central nervous system (CNS) (1). Myelin basic protein (MBP) is an abundant CNS myelin membrane protein that plays an important role in nerve myelination. MBP helps to adhere the cytoplasmic leaflets of adjacent oligodendrocyte membranes to one another (2). CNPase (2', 3'-cyclic nucleotide 3'-phosphodiesterase) is an enzyme highly expressed in oligodendrocytes and accounts for roughly 4% of the total myelin protein in the CNS (3). CNPase binds to tubulin heterodimers and plays a role in tubulin polymerization, and oligodendrocyte process outgrowth (4). Myelin proteolipid protein (PLP1) corresponds to the majority of myelin proteins in the CNS, providing support to axons and modulating axonal growth (5). Myelin-associated glycoprotein (MAG) is localized in oligodendroglial membranes of myelin sheaths where it plays a role in interaction between axons and glia, and has been shown to promote axonal protective effects (6,7). Chondroitin sulfate proteoglycan 4 (CSP4, NG2) is a type I membrane glycoprotein expressed by oligodendroglial precursor cells (OPCs) that plays a role in cell attachment and migration (8). SRY-box 10 (Sox10) is a high-mobility group transcription factor expressed throughout oligodendrocyte development required for myelin gene expression (9,10). Myelin-oligodendrocyte glycoprotein (MOG) is a type I membrane bound glycoprotein of the immunoglobulin superfamily that is enriched in the outer lamella of the myelin sheath (11). Olig2 is a basic helix–loop–helix (bHLH) transcription factor necessary for oligodendrocyte and motor neuron differentiation and development in the CNS (12,13). Olig2 has been shown to be a useful marker for primary and mature oligodendrocytes and is universally expressed in diffuse gliomas (14,15).
  1. Bradl, M. and Lassmann, H. (2010) Acta Neuropathol 119, 37-53.
  2. Harauz, G. and Boggs, J.M. (2013) J Neurochem 125, 334-61.
  3. Kozlov, G. et al. (2003) J Biol Chem 278, 46021-8.
  4. Lee, J. et al. (2005) J Cell Biol 170, 661-73.
  5. Thomson, C.E. et al. Dev Neurosci 27, 27-36.
  6. Quarles, R.H. (2007) J Neurochem 100, 1431-48.
  7. Nguyen, T. et al. (2009) J Neurosci 29, 630-7.
  8. Karram, K. et al. (2005) J Anat 207, 735-44.
  9. Küspert, M. et al. (2011) Nucleic Acids Res 39, 1280-93.
  10. Stolt, C.C. et al. (2002) Genes Dev 16, 165-70.
  11. Johns, T.G. and Bernard, C.C. (1999) J Neurochem 72, 1-9.
  12. Zhou, Q. et al. (2000) Neuron 25, 331-43.
  13. Takebayashi, H. et al. (2002) Curr Biol 12, 1157-63.
  14. Ligon, K.L. et al. (2004) J Neuropathol Exp Neurol 63, 499-509.
  15. Yokoo, H. et al. (2004) Am J Pathol 164, 1717-25.

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