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8678
Oncogene and Tumor Suppressor Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Oncogene and Tumor Suppressor Antibody Sampler Kit #8678

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Product Description

The Oncogenes and Tumor Suppressor Antibody Sampler Kit offers an economical means of investigating proteins commonly involved in the biological pathways behind oncogenesis, tumor metastasis, and cancer pathology. The kit contains enough primary and secondary antibody to perform four western blot experiments with each antibody.

Specificity / Sensitivity

Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb recognizes endogenous levels of Akt only when phosphorylated at Ser473. BRCA1 Antibody recognizes endogenous levels of total BRCA1 protein. The antibody detects BRAC1 nuclear isoforms 1, 2, and 4, but not BRAC1 cytoplasmic isoforms 3 and 5, and does not detect BRCA2. E-Cadherin (24E10) Rabbit mAb recognizes endogenous levels of total E-cadherin protein. The antibody does not cross-react with related family members, such as N-cadherin. Phospho-Estrogen Receptor α (Ser167) (D1A3) Rabbit mAb recognizes endogeneous levels of ERα only when phosphorylated at Ser167. The antibody cross reacts with a nonspecific band at around 77 kDa. HER2/ErbB2 (D8F12) XP® Rabbit mAb recognizes endogenous levels of total HER2/ErbB2 protein. p53 (7F5) Rabbit mAb detects endogenous levels of total p53 protein. The antibody binding has been mapped to the amino terminus region of human p53 protein. PTEN (138G6) Rabbit mAb recognizes endogenous levels of total PTEN protein. Stathmin Antibody recognizes endogenous levels of total stathmin protein. The antibody does not cross-react with related proteins, such as SCG10, SCLIP, and RB3.

Source / Purification

Phospho-specific monoclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser473 of human Akt protein or Ser167 of human estrogen receptor α protein. Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro780 of human E-cadherin protein, residues near the amino terminus of human HER2/ErbB2 protein, the carboxy terminus of human PTEN protein, or with a full-length human p53 fusion protein. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the amino terminus of human BRCA1 protein or residues surrounding Ser38 of human stathmin protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Background

Oncogenesis is a multistep process leading to sequential alterations in several oncogenes, tumor-suppressor genes, and microRNA genes (1,2). These alterations often disrupt the expression, function, and/or activity of proteins regulating cell growth and programmed cell death. Many of the molecular mechanisms and biological pathways driving oncogenesis and cancer pathology have been identified. The signal transduction pathways regulating apoptosis, cell-cycle progression, cell adhesion, cell migration, and DNA damage responses are often disrupted. HER2/ErbB2 (3), E-Cadherin (4), p53 (5,6), Stathmin (7), BRCA1 (8,9), Akt (10), PTEN (11), and Estrogen Receptor α (12) function in many of these pathways.

  1. Berger, A.H. et al. (2011) Nature 476, 163-9.
  2. Peltomäki, P. (2012) Exp Cell Res 318, 299-310.
  3. Dittadi, R. and Gion, M. (2000) J Natl Cancer Inst 92, 1443-4.
  4. Hazan, R.B. et al. (2004) Ann N Y Acad Sci 1014, 155-63.
  5. Rahman, N. and Stratton, M.R. (1998) Annu Rev Genet 32, 95-121.
  6. Freed-Pastor, W.A. and Prives, C. (2012) Genes Dev 26, 1268-86.
  7. Belletti, B. and Baldassarre, G. (2011) Expert Opin Ther Targets 15, 1249-66.
  8. Gayther, S.A. et al. (1999) Am J Hum Genet 65, 1021-9.
  9. Scully, R. and Livingston, D.M. (2000) Nature 408, 429-32.
  10. Jazirehi, A.R. et al. (2012) Am J Cancer Res 2, 178-91.
  11. Saal, L.H. et al. (2008) Nat Genet 40, 102-7.
  12. Pópulo, H. et al. (2012) Int J Mol Sci 13, 1886-918.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.