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PhosphoSitePlus® Resource

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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
E-Cadherin (24E10) Rabbit mAb 3195 20 µl
Western Blotting Immunohistochemistry Immunofluorescence Flow Cytometry
H M 135 Rabbit IgG
p53 (7F5) Rabbit mAb 2527 20 µl
Western Blotting Immunohistochemistry Immunofluorescence Flow Cytometry Chromatin Immunoprecipitation
H Mk 53 Rabbit IgG
Stathmin Antibody 3352 20 µl
Western Blotting Immunohistochemistry
H M R Mk 19 Rabbit 
BRCA1 Antibody 9010 20 µl
Western Blotting Immunoprecipitation
H 220 Rabbit 
Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb 4060 20 µl
Western Blotting Immunoprecipitation Immunohistochemistry Immunofluorescence Flow Cytometry
H M R Hm Mk Dm Z B 60 Rabbit IgG
PTEN (138G6) Rabbit mAb 9559 20 µl
Western Blotting Immunoprecipitation Immunohistochemistry
H M R Mk 54 Rabbit IgG
Phospho-Estrogen Receptor α (Ser167) (D1A3) Rabbit mAb 5587 20 µl
Western Blotting
H 66 Rabbit IgG
HER2/ErbB2 (D8F12) XP® Rabbit mAb 4290 20 µl
Western Blotting Immunohistochemistry
H M 185 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting
Goat 

Product Description

The Oncogenes and Tumor Suppressor Antibody Sampler Kit offers an economical means of investigating proteins commonly involved in the biological pathways behind oncogenesis, tumor metastasis, and cancer pathology. The kit contains enough primary and secondary antibody to perform four western blot experiments with each antibody.


Specificity / Sensitivity

Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb recognizes endogenous levels of Akt only when phosphorylated at Ser473. BRCA1 Antibody recognizes endogenous levels of total BRCA1 protein. The antibody detects BRAC1 nuclear isoforms 1, 2, and 4, but not BRAC1 cytoplasmic isoforms 3 and 5, and does not detect BRCA2. E-Cadherin (24E10) Rabbit mAb recognizes endogenous levels of total E-cadherin protein. The antibody does not cross-react with related family members, such as N-cadherin. Phospho-Estrogen Receptor α (Ser167) (D1A3) Rabbit mAb recognizes endogeneous levels of ERα only when phosphorylated at Ser167. The antibody cross reacts with a nonspecific band at around 77 kDa. HER2/ErbB2 (D8F12) XP® Rabbit mAb recognizes endogenous levels of total HER2/ErbB2 protein. p53 (7F5) Rabbit mAb detects endogenous levels of total p53 protein. The antibody binding has been mapped to the amino terminus region of human p53 protein. PTEN (138G6) Rabbit mAb recognizes endogenous levels of total PTEN protein. Stathmin Antibody recognizes endogenous levels of total stathmin protein. The antibody does not cross-react with related proteins, such as SCG10, SCLIP, and RB3.


Source / Purification

Phospho-specific monoclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser473 of human Akt protein or Ser167 of human estrogen receptor α protein. Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro780 of human E-cadherin protein, residues near the amino terminus of human HER2/ErbB2 protein, the carboxy terminus of human PTEN protein, or with a full-length human p53 fusion protein. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the amino terminus of human BRCA1 protein or residues surrounding Ser38 of human stathmin protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Oncogenesis is a multistep process leading to sequential alterations in several oncogenes, tumor-suppressor genes, and microRNA genes (1,2). These alterations often disrupt the expression, function, and/or activity of proteins regulating cell growth and programmed cell death. Many of the molecular mechanisms and biological pathways driving oncogenesis and cancer pathology have been identified. The signal transduction pathways regulating apoptosis, cell-cycle progression, cell adhesion, cell migration, and DNA damage responses are often disrupted. HER2/ErbB2 (3), E-Cadherin (4), p53 (5,6), Stathmin (7), BRCA1 (8,9), Akt (10), PTEN (11), and Estrogen Receptor α (12) function in many of these pathways.


1.  Berger, A.H. et al. (2011) Nature 476, 163-9.

2.  Rahman, N. and Stratton, M.R. (1998) Annu Rev Genet 32, 95-121.

3.  Peltomäki, P. (2012) Exp Cell Res 318, 299-310.

4.  Gayther, S.A. et al. (1999) Am J Hum Genet 65, 1021-9.

5.  Dittadi, R. and Gion, M. (2000) J Natl Cancer Inst 92, 1443-4.

6.  Hazan, R.B. et al. (2004) Ann N Y Acad Sci 1014, 155-63.

7.  Scully, R. and Livingston, D.M. (2000) Nature 408, 429-32.

8.  Freed-Pastor, W.A. and Prives, C. (2012) Genes Dev 26, 1268-86.

9.  Belletti, B. and Baldassarre, G. (2011) Expert Opin Ther Targets 15, 1249-66.

10.  Jazirehi, A.R. et al. (2012) Am J Cancer Res 2, 178-91.

11.  Saal, L.H. et al. (2008) Nat Genet 40, 102-7.

12.  Pópulo, H. et al. (2012) Int J Mol Sci 13, 1886-918.


Entrez-Gene Id 207 , 208 , 10000 , 672 , 999 , 2099 , 2064 , 7157 , 5728 , 3925
Swiss-Prot Acc. P31749 , P31751 , Q9Y243 , P38398 , P12830 , P03372 , P04626 , P04637 , P60484 , P16949


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 5,675,063.

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Oncogene and Tumor Suppressor Antibody Sampler Kit