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8678
Oncogene and Tumor Suppressor Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Oncogene and Tumor Suppressor Antibody Sampler Kit #8678

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Oncogene and Tumor Suppressor Antibody Sampler Kit: Image 1
Western blot analysis of extracts from 293 and COS cells, using p53 (7F5) Rabbit mAb.
Oncogene and Tumor Suppressor Antibody Sampler Kit: Image 2
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using p53 (7F5) Rabbit mAb.
Oncogene and Tumor Suppressor Antibody Sampler Kit: Image 3
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using p53 (7F5) Rabbit mAb.
Oncogene and Tumor Suppressor Antibody Sampler Kit: Image 4
Immunohistochemical analysis of paraffin-embedded HT-29 (left) and SaOs-2 (right) cells, using p53 (7F5) Rabbit mAb. Note the lack of staining in p53-negative SaOs-2 cells.
Oncogene and Tumor Suppressor Antibody Sampler Kit: Image 5
Confocal Immunofluorescent analysis of HT-29 cells using p53 (7F5) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).
Oncogene and Tumor Suppressor Antibody Sampler Kit: Image 6
Flow cytometric analysis of HT-29 cells using p53 (7F5) Rabbit mAb (solid line) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed line). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Oncogene and Tumor Suppressor Antibody Sampler Kit: Image 7
Chromatin immunoprecipitations were performed with cross-linked chromatin from HCT116 cells treated with UV (100 J/m2 followed by a 3 hour recovery) and either p53 (7F5) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human CDKN1A Promoter Primers #6449, human MDM2 intron 2 primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
Oncogene and Tumor Suppressor Antibody Sampler Kit: Image 8
Western blot analysis of extracts from various cell lines, using E-Cadherin (24E10) Rabbit mAb.
Oncogene and Tumor Suppressor Antibody Sampler Kit: Image 9
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using E-Cadherin (24E10) Rabbit mAb.
Oncogene and Tumor Suppressor Antibody Sampler Kit: Image 10
Immunohistochemical analysis of paraffin-embedded human metastatic adenocarcinoma in lymph node, using E-Cadherin (24E10) Rabbit mAb.
Oncogene and Tumor Suppressor Antibody Sampler Kit: Image 11
Immunohistochemical analysis of paraffin-embedded mouse lung using E-Cadherin (24E10) Rabbit mAb.
Oncogene and Tumor Suppressor Antibody Sampler Kit: Image 12
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using E-Cadherin (24E10) Rabbit mAb in the presence of control peptide (left) or E-Cadherin Blocking Peptide #1050 (right).
Oncogene and Tumor Suppressor Antibody Sampler Kit: Image 13
Confocal immunofluorescent images of MCF7 cells using E-Cadherin (24E10) Rabbit mAb (green, left) compared to an isotype control (right). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).
Oncogene and Tumor Suppressor Antibody Sampler Kit: Image 14
Flow cytometric analysis of fixed and permeabilized HeLa cells (blue, negative) and MCF7 cells (green, positive) using E-Cadherin (24E10) Rabbit mAb.
Oncogene and Tumor Suppressor Antibody Sampler Kit: Image 15
Western blot analysis of extracts from C6, SH-SY5Y, SK-N-MC, PC12, COS and HeLa cells, using Stathmin Antibody.
Oncogene and Tumor Suppressor Antibody Sampler Kit: Image 16
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic localization, using Stathmin Antibody.
Oncogene and Tumor Suppressor Antibody Sampler Kit: Image 17
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Stathmin Antibody.
Oncogene and Tumor Suppressor Antibody Sampler Kit: Image 18
Western blot analysis of extracts from PC-3 cells, untreated or LY294002/wortmannin-treated, and NIH/3T3 cells, serum-starved or PDGF-treated, using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (upper) or Akt (pan) (C67E7) Rabbit mAb #4691 (lower).
Oncogene and Tumor Suppressor Antibody Sampler Kit: Image 19
Immunoprecipitation of phospho-Akt (Ser473) from Jurkat extracts treated with Calyculin A #9902 (100nM, 30 min). Lane 1 is 10% input, lane 2 is Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb, and lane 3 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900. Western blot analysis was performed with Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody.
Oncogene and Tumor Suppressor Antibody Sampler Kit: Image 20
Immunohistochemical analysis of paraffin-embedded MDA-MB-468 xenograft using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (left) or PTEN (138G6) Rabbit mAb #9559 (right). Note the presence of P-Akt staining in the PTEN deficient MDA-MB-468 cells.
Oncogene and Tumor Suppressor Antibody Sampler Kit: Image 21
Immunohistochemical analysis of paraffin-embedded human breast carcinoma comparing SignalStain® Antibody Diluent #8112 (left) to TBST/5% normal goat serum (right) using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060.
Oncogene and Tumor Suppressor Antibody Sampler Kit: Image 22
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb.
Oncogene and Tumor Suppressor Antibody Sampler Kit: Image 23
Immunohistochemical analysis using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb on SignalSlide® Phospho-Akt (Ser473) IHC Controls #8101 (paraffin-embedded LNCaP cells, untreated (left) or LY294002-treated (right)).
Oncogene and Tumor Suppressor Antibody Sampler Kit: Image 24
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb.
Oncogene and Tumor Suppressor Antibody Sampler Kit: Image 25
Immunohistochemical analysis of paraffin-embedded PTEN heterozygous mutant mouse endometrium using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb. (Tissue section courtesy of Dr. Sabina Signoretti, Brigham and Women's Hospital, Harvard Medical School, Boston, MA.)
Oncogene and Tumor Suppressor Antibody Sampler Kit: Image 26
Immunohistochemical analysis of paraffin-embedded U-87MG xenograft, untreated (left) or lambda phosphatase-treated (right), using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb.
Oncogene and Tumor Suppressor Antibody Sampler Kit: Image 27
Confocal immunofluorescent analysis of C2C12 cells, LY294002-treated (left) or insulin-treated (right), using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin #8953 (red). Blue pseudocolor = DRAQ5®#4084 (fluorescent DNA dye).
Oncogene and Tumor Suppressor Antibody Sampler Kit: Image 28
Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002 #9901, Wortmannin #9951, and U0126 #9903 (50 μM, 1 μM, and 10 μM, 2 hr; blue) using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Oncogene and Tumor Suppressor Antibody Sampler Kit: Image 29
Western blot analysis of extracts from SK-BR-3 and MCF7 cells using HER2/ErbB2 (D8F12) XP® Rabbit mAb.
Oncogene and Tumor Suppressor Antibody Sampler Kit: Image 30
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma using HER2/ErbB2 (D8F12) XP® Rabbit mAb performed on the Leica® BOND™ Rx. 
Oncogene and Tumor Suppressor Antibody Sampler Kit: Image 31
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma using HER2/ErbB2 (D8F12) XP® Rabbit mAb performed on the Leica® BOND™ Rx. 
Oncogene and Tumor Suppressor Antibody Sampler Kit: Image 32
Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma using HER2/ErbB2 (D8F12) XP® Rabbit mAb performed on the Leica® BOND™ Rx. 
Oncogene and Tumor Suppressor Antibody Sampler Kit: Image 33
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using HER2/ErbB2 (D8F12) XP® Rabbit mAb performed on the Leica® BOND™ Rx. 
Oncogene and Tumor Suppressor Antibody Sampler Kit: Image 34
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using HER2/ErbB2 (D8F12) XP® Rabbit mAb.
Oncogene and Tumor Suppressor Antibody Sampler Kit: Image 35
Immunohistochemical analysis of paraffin-embedded human ductal breast carcinoma using HER2/ErbB2 (D8F12) XP® Rabbit mAb.
Oncogene and Tumor Suppressor Antibody Sampler Kit: Image 36
Immunohistochemical analysis of paraffin-embedded SK-BR-3 (Her2 high, left) and MCF7 cell pellets (Her2 low, right) using HER2/ErbB2 (D8F12) XP® Rabbit mAb.
Oncogene and Tumor Suppressor Antibody Sampler Kit: Image 37
Western analysis of extracts from MCF7 cells, untreated or treated with Estrodiol/EGF (100nM each, together for 30 min) and further treated with calf intestinal phosphatase (CIP), using Phospho-Estrogen Receptor α (Ser167) (D1A3) Rabbit mAb (upper) or Estrogen Receptor α (D8H8) Rabbit mAb #8644 (lower).
Oncogene and Tumor Suppressor Antibody Sampler Kit: Image 38
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
Oncogene and Tumor Suppressor Antibody Sampler Kit: Image 39
Western blot analysis of untreated and UV-treated (50 mJ/cm2, 30 min) HeLa cells, using BRCA1 antibody.
Oncogene and Tumor Suppressor Antibody Sampler Kit: Image 40
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence® PTEN siRNA I (+), using PTEN Antibody #9552 and p42 MAPK (Erk2) Antibody #9108. PTEN Antibody confirms silencing of PTEN expression, while the p42 MAPK (Erk2) Antibody is used to control for loading and specificity of PTEN siRNA.
Oncogene and Tumor Suppressor Antibody Sampler Kit: Image 41
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence® PTEN siRNA II (+), using PTEN (138G6) Rabbit mAb and β-Actin (13E5) Rabbit mAb #4970. PTEN (138G6) Rabbit mAb confirms silencing of PTEN expression, while the β-Actin (13E5) Rabbit mAb is used to control for loading and specificity of PTEN siRNA.
Oncogene and Tumor Suppressor Antibody Sampler Kit: Image 42
Western blot analysis of extracts from various cell lines using PTEN (138G6) Rabbit mAb.
Oncogene and Tumor Suppressor Antibody Sampler Kit: Image 43
Immunohistochemical analysis of paraffin-embedded MDA-MB-468 xenograft, using Phospho-Akt (Ser473) (736E11) Rabbit mAb (IHC Preferred) (#3787) (left) or PTEN (138G6) Rabbit mAb (right). MDA-MB-468 cells lack PTEN. Note the lack of PTEN staining in the Phospho-Akt positive cells.
Oncogene and Tumor Suppressor Antibody Sampler Kit: Image 44
Immunohistochemical analysis of paraffin-embedded human lung carcinoma (left) and prostate carcinoma (right), using PTEN (138G6) Rabbit mAb. Note the stromal cell staining in the PTEN negative lung carcinoma, and the cancer cell staining in the PTEN positive prostate carcinoma.
Oncogene and Tumor Suppressor Antibody Sampler Kit: Image 45
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using PTEN (138G6) Rabbit mAb in the presence of control peptide (left) or PTEN Blocking Peptide #1250 (right).
Oncogene and Tumor Suppressor Antibody Sampler Kit: Image 46
Immunohistochemical analysis of paraffin-embedded cell pellets demonstrating the specificity of PTEN (138G6) Rabbit mAb: DU145, HT-29 and MCF-7 (PTEN positive) and Jurkat, MDA-MB-468 and LNCaP (PTEN negative).
Oncogene and Tumor Suppressor Antibody Sampler Kit: Image 47
Immunohistochemical analysis of paraffin-embedded xenografts using PTEN (138G6) Rabbit mAb. DU145 (left) and A549 (middle) are PTEN positive cell lines, while U-87MG (right) is PTEN negative.
Oncogene and Tumor Suppressor Antibody Sampler Kit: Image 48
Immunohistochemical analysis of paraffin-embedded MDA-MB-468 xenograft using Phospho-Akt (Ser473) (D9E) Rabbit mAb #4060 (left) or PTEN (138G6) Rabbit mAb (right). Note the presence of P-Akt staining in the PTEN deficient MDA-MB-468 cells.
Oncogene and Tumor Suppressor Antibody Sampler Kit: Image 49
Immunohistochemical analysis of paraffin-embedded human ovarian serous carcinoma using PTEN (138G6) Rabbit mAb.
Oncogene and Tumor Suppressor Antibody Sampler Kit: Image 50
Immunohistochemical analysis of paraffin-embedded human ovarian clear cell carcinoma using PTEN (138G6) Rabbit mAb.
Inquiry Info.# 8678

Product Description

The Oncogenes and Tumor Suppressor Antibody Sampler Kit offers an economical means of investigating proteins commonly involved in the biological pathways behind oncogenesis, tumor metastasis, and cancer pathology. The kit contains enough primary and secondary antibody to perform four western blot experiments with each antibody.

Specificity / Sensitivity

Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb recognizes endogenous levels of Akt only when phosphorylated at Ser473. BRCA1 Antibody recognizes endogenous levels of total BRCA1 protein. The antibody detects BRAC1 nuclear isoforms 1, 2, and 4, but not BRAC1 cytoplasmic isoforms 3 and 5, and does not detect BRCA2. E-Cadherin (24E10) Rabbit mAb recognizes endogenous levels of total E-cadherin protein. The antibody does not cross-react with related family members, such as N-cadherin. Phospho-Estrogen Receptor α (Ser167) (D1A3) Rabbit mAb recognizes endogeneous levels of ERα only when phosphorylated at Ser167. The antibody cross reacts with a nonspecific band at around 77 kDa. HER2/ErbB2 (D8F12) XP® Rabbit mAb recognizes endogenous levels of total HER2/ErbB2 protein. p53 (7F5) Rabbit mAb detects endogenous levels of total p53 protein. The antibody binding has been mapped to the amino terminus region of human p53 protein. PTEN (138G6) Rabbit mAb recognizes endogenous levels of total PTEN protein. Stathmin Antibody recognizes endogenous levels of total stathmin protein. The antibody does not cross-react with related proteins, such as SCG10, SCLIP, and RB3.

Source / Purification

Phospho-specific monoclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser473 of human Akt protein or Ser167 of human estrogen receptor α protein. Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro780 of human E-cadherin protein, residues near the amino terminus of human HER2/ErbB2 protein, the carboxy terminus of human PTEN protein, or with a full-length human p53 fusion protein. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the amino terminus of human BRCA1 protein or residues surrounding Ser38 of human stathmin protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Background

Oncogenesis is a multistep process leading to sequential alterations in several oncogenes, tumor-suppressor genes, and microRNA genes (1,2). These alterations often disrupt the expression, function, and/or activity of proteins regulating cell growth and programmed cell death. Many of the molecular mechanisms and biological pathways driving oncogenesis and cancer pathology have been identified. The signal transduction pathways regulating apoptosis, cell-cycle progression, cell adhesion, cell migration, and DNA damage responses are often disrupted. HER2/ErbB2 (3), E-Cadherin (4), p53 (5,6), Stathmin (7), BRCA1 (8,9), Akt (10), PTEN (11), and Estrogen Receptor α (12) function in many of these pathways.
  1. Berger, A.H. et al. (2011) Nature 476, 163-9.
  2. Peltomäki, P. (2012) Exp Cell Res 318, 299-310.
  3. Dittadi, R. and Gion, M. (2000) J Natl Cancer Inst 92, 1443-4.
  4. Hazan, R.B. et al. (2004) Ann N Y Acad Sci 1014, 155-63.
  5. Rahman, N. and Stratton, M.R. (1998) Annu Rev Genet 32, 95-121.
  6. Freed-Pastor, W.A. and Prives, C. (2012) Genes Dev 26, 1268-86.
  7. Belletti, B. and Baldassarre, G. (2011) Expert Opin Ther Targets 15, 1249-66.
  8. Gayther, S.A. et al. (1999) Am J Hum Genet 65, 1021-9.
  9. Scully, R. and Livingston, D.M. (2000) Nature 408, 429-32.
  10. Jazirehi, A.R. et al. (2012) Am J Cancer Res 2, 178-91.
  11. Saal, L.H. et al. (2008) Nat Genet 40, 102-7.
  12. Pópulo, H. et al. (2012) Int J Mol Sci 13, 1886-918.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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