WB
H M R Hm
Endogenous
100, 95 (Mouse)
Rat IgG1
#Q13415
4998
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Hamster
Source / Purification
Monoclonal antibody is produced by immunizing animals with a His6-tagged recombinant human ORC1 protein.
Background
The origin recognition complex (ORC) is a highly conserved heterohexameric protein complex that associates with DNA at or near initiation of DNA replication sites. All six ORC subunits are essential for initiation of DNA replication (1-3), and ORC may be involved in regulation of gene expression in response to stress (4). ORC binding to DNA permits the ordered binding of other proteins such as cdc6 and MCMs to form pre-replication complexes (Pre-RCs). Pre-RCs form between telophase and early G1 phase of the cell cycle and are inactivated at the onset of DNA synthesis, allowing coordinated regulation of DNA replication and cell division (5). Modification of one or more of the six ORC subunits may be responsible for its inactivation during S phase, but the chromatin binding behavior of the ORC subunits during the cell division cycle is still under investigation (6-7).
- Machida, Y.J. et al. (2005) J. Biol. Chem. 280, 27624-27630.
- Baltin, J. et al. (2006) J. Biol. Chem. 281, 12428-12435.
- Gibson, D.G. et al. (2006) Genes Cells 11, 557-573.
- Ramachandran, L. et al. (2006) FEMS Yeast Res. 6, 763-776.
- Rowles, A. and Blow, J.J. (1997) Curr. Opin. Genet. Dev. 7, 152-157.
- DePamphilis, M.L. (2003) Gene 310, 1-15.
- McNairn, A.J. et al. (2005) Exp. Cell. Res. 308, 345-356.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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