WB
H
Endogenous
42
Rabbit IgG
#Q9UN81
4029
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly162 of human ORF1p protein.
Background
LINE-1, also known as L1, is a non-long terminal repeat (non-LTR) retrotransposon with hundreds of thousands of copies in the human genome (1, 2). Like all non-LTRs, L1 replicates by target-primed reverse transcription (TPRT) (3). The L1 retrotransposon encodes two proteins critical to this process: ORF1p and ORF2p. ORF2p contributes to endonuclease and reverse transcriptase activity, while ORF1p acts as a nucleic acid chaperone that binds RNA (4-8). Many types of cancers have been shown to have L1 insertions within tumor suppressor genes, disrupting their expression and contributing to tumorigenesis (9).
While normally silenced, ORF1p is expressed in many types of cancers (9-12). Recently, it has been found that ORF1p can become phosphorylated and that this event impacts its ability to promote retrotransposition. This creates a novel dynamic to which the host could actively affect L1 replication through various signaling cascades (13).
- Kazazian, H.H. and Moran, J.V. (1998) Nat Genet 19, 19-24.
- Malik, H.S. et al. (1999) Mol Biol Evol 16, 793-805.
- Wilhelm, M. and Wilhelm, F.X. (2001) Cell Mol Life Sci 58, 1246-62.
- Scott, A.F. et al. (1987) Genomics 1, 113-25.
- Moran, J.V. et al. (1996) Cell 87, 917-27.
- Feng, Q. et al. (1996) Cell 87, 905-16.
- Martin, S.L. and Bushman, F.D. (2001) Mol Cell Biol 21, 467-75.
- Kolosha, V.O. and Martin, S.L. (1997) Proc Natl Acad Sci U S A 94, 10155-60.
- Lee, E. et al. (2012) Science 337, 967-71.
- Rodić, N. et al. (2015) Nat Med 21, 1060-4.
- Doucet-O'Hare, T.T. et al. (2015) Proc Natl Acad Sci U S A 112, E4894-900.
- Chen, L. et al. (2012) Breast Cancer Res Treat 136, 129-42.
- Rodić, N. et al. (2014) Am J Pathol 184, 1280-6.
- Cook, P.R. et al. (2015) Proc Natl Acad Sci U S A 112, 4298-303.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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