Revision 4
#88701
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W, W-S
Reactivity:
H
Sensitivity:
Endogenous
MW (kDa):
42
Source/Isotype:
Rabbit IgG
UniProt ID:
#Q9UN81
Entrez-Gene Id:
4029
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:10 - 1:50 |
Storage
Specificity/Sensitivity
Source / Purification
Background
While normally silenced, ORF1p is expressed in many types of cancers (9-12). Recently, it has been found that ORF1p can become phosphorylated and that this event impacts its ability to promote retrotransposition. This creates a novel dynamic to which the host could actively affect L1 replication through various signaling cascades (13).
Background References
- Kazazian, H.H. and Moran, J.V. (1998) Nat Genet 19, 19-24.
- Malik, H.S. et al. (1999) Mol Biol Evol 16, 793-805.
- Wilhelm, M. and Wilhelm, F.X. (2001) Cell Mol Life Sci 58, 1246-62.
- Scott, A.F. et al. (1987) Genomics 1, 113-25.
- Moran, J.V. et al. (1996) Cell 87, 917-27.
- Feng, Q. et al. (1996) Cell 87, 905-16.
- Martin, S.L. and Bushman, F.D. (2001) Mol Cell Biol 21, 467-75.
- Kolosha, V.O. and Martin, S.L. (1997) Proc Natl Acad Sci U S A 94, 10155-60.
- Lee, E. et al. (2012) Science 337, 967-71.
- Rodić, N. et al. (2015) Nat Med 21, 1060-4.
- Doucet-O'Hare, T.T. et al. (2015) Proc Natl Acad Sci U S A 112, E4894-900.
- Chen, L. et al. (2012) Breast Cancer Res Treat 136, 129-42.
- Rodić, N. et al. (2014) Am J Pathol 184, 1280-6.
- Cook, P.R. et al. (2015) Proc Natl Acad Sci U S A 112, 4298-303.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting W-S: Simple Western™
Cross-Reactivity Key
H: Human
Trademarks and Patents
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