Revision 2

#8653Store at -20C

1 Kit

(5 x 20 microliters)

Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected] cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Product Includes Product # Quantity Mol. Wt Isotype/Source
AIF (D39D2) XP® Rabbit mAb 5318 20 µl 67 kDa Rabbit IgG
EEA1 (C45B10) Rabbit mAb 3288 20 µl 170 kDa Rabbit IgG
LAMP1 (D2D11) XP® Rabbit mAb 9091 20 µl 42 (non-glycosylated), 90-120 (glycosylated) kDa Rabbit IgG
PDI (C81H6) Rabbit mAb 3501 20 µl 57 kDa Rabbit 
RCAS1 (D2B6N) XP® Rabbit mAb 12290 20 µl 32 kDa Rabbit IgG

Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.

Description

The Organelle Localization IF Antibody Sampler Kit provides an economical means for identification of cellular organelles by fluorescence immunocytochemistry (IF-IC). This kit includes enough primary antibody to perform at least twenty IF-IC tests or two western blots with each antibody.

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Background

Knowledge of the subcellular location of a protein may reveal its potential role in a variety of cellular processes. One can confirm the subcellular location of a marker that colocalizes with one of the organelle-specific antibodies in this kit. While these antibodies serve as powerful tools for immunofluorescence, they may also be used as western blot controls for fractionated cell lysates.

Background References

    Trademarks and Patents

    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.
    All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

    Limited Uses

    Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    Revision 2
    #8653

    Organelle Localization IF Antibody Sampler Kit

    Organelle Localization IF Antibody Sampler Kit: Image 1 Expand Image
    Western blot analysis of extracts from various cell lines using RCAS1 (D2B6N) XP® Rabbit mAb.
    Organelle Localization IF Antibody Sampler Kit: Image 2 Expand Image
    Western blot analysis of extracts from various cell lines using EEA1 (C45B10) Rabbit mAb.
    Organelle Localization IF Antibody Sampler Kit: Image 3 Expand Image
    Confocal immunofluorescent tile scan of a fixed frozen brain from an amyloid mouse model of Alzheimer's disease using EEA1 (C45B10) Rabbit mAb #3288 (green), GFAP (GA5) Mouse mAb #3670 (red), and DAPI #4083 (blue).
    Organelle Localization IF Antibody Sampler Kit: Image 4 Expand Image
    Confocal immunofluorescent analysis of fixed frozen mouse thalamus, labeled with EEA1 (C45B10) Rabbit mAb #3288 (left, green) and co-labeled with GFAP (GA5) Mouse mAb #3670 (right, red) and DAPI #4083 (right, blue).
    Organelle Localization IF Antibody Sampler Kit: Image 5 Expand Image
    Confocal immunofluorescent analysis of fixed frozen mouse hippocampus, labeled with EEA1 (C45B10) Rabbit mAb #3288 (left, green) and co-labeled with GFAP (GA5) Mouse mAb #3670 (right, red) and DAPI #4093 (right, blue).
    Organelle Localization IF Antibody Sampler Kit: Image 6 Expand Image
    Western blot analysis of extracts from various cell types using PDI (C81H6) Rabbit mAb.
    Organelle Localization IF Antibody Sampler Kit: Image 7 Expand Image
    Western blot analysis of extracts from Jurkat, C2C12, and C6 cells using AIF (D39D2) XP® Rabbit mAb.
    Organelle Localization IF Antibody Sampler Kit: Image 8 Expand Image
    Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using AIF (D39D2) XP® Rabbit mAb.
    Organelle Localization IF Antibody Sampler Kit: Image 9 Expand Image
    Western blot analysis of extracts from various cell lines using LAMP1 (D2D11) XP® Rabbit mAb.
    Organelle Localization IF Antibody Sampler Kit: Image 10 Expand Image
    Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human RCAS1 (hRCAS1-Myc/DDK; +), using RCAS1 (D2B6N) XP® Rabbit mAb.
    Organelle Localization IF Antibody Sampler Kit: Image 11 Expand Image
    Confocal immunofluorescent analysis of HeLa cells using EEA1 (C45B10) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5 (fluorescent DNA dye).
    Organelle Localization IF Antibody Sampler Kit: Image 12 Expand Image
    Immunohistochemical analysis of paraffin-embedded human lung carcinoma using PDI (C81H6) Rabbit mAb.
    Organelle Localization IF Antibody Sampler Kit: Image 13 Expand Image
    Immunohistochemical analysis of paraffin-embedded human papillary thyroid carcinoma using AIF (D39D2) XP® Rabbit mAb.
    Organelle Localization IF Antibody Sampler Kit: Image 14 Expand Image
    Immunohistochemical analysis of normal human kidney using LAMP1 (D2D11) XP® Rabbit mAb.
    Organelle Localization IF Antibody Sampler Kit: Image 15 Expand Image
    Immunoprecipitation of RCAS1 from HCT 116 extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or RCAS1 (D2B6N) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using RCAS1 (D2B6N) XP® Rabbit mAb.
    Organelle Localization IF Antibody Sampler Kit: Image 16 Expand Image
    Immunohistochemical analysis of paraffin-embedded human lymphoma using PDI (C81H6) Rabbit mAb.
    Organelle Localization IF Antibody Sampler Kit: Image 17 Expand Image
    Confocal immunofluorescent analysis of mouse brainstem using AIF (D39D2) XP® Rabbit mAb #5318 (green) and GFAP (GA5) Mouse mAb #3670 (red). Sections were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
    Organelle Localization IF Antibody Sampler Kit: Image 18 Expand Image
    Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using AIF (D39D2) XP® Rabbit mAb.
    Organelle Localization IF Antibody Sampler Kit: Image 19 Expand Image
    Immunohistochemical analysis of human lung carcinoma using LAMP1 (D2D11) XP® Rabbit mAb.
    Organelle Localization IF Antibody Sampler Kit: Image 20 Expand Image
    Confocal immunofluorescent analysis of MCF7 cells, untreated (left) or treated with Brefeldin A #9972 (5 μg/ml, 1 hr; right), using RCAS1 (D2B6N) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
    Organelle Localization IF Antibody Sampler Kit: Image 21 Expand Image
    Immunohistochemical analysis of paraffin-embedded mouse spleen using PDI (C81H6) Rabbit mAb.
    Organelle Localization IF Antibody Sampler Kit: Image 22 Expand Image
    Confocal immunofluorescent analysis of mouse cerebellum using AIF (D39D2) XP® Rabbit mAb #5318 (green), GFAP (GA5) Mouse mAb #3670 (red), and F4/80 (BM8.1) Rat mAb #71299 (blue).
    Organelle Localization IF Antibody Sampler Kit: Image 23 Expand Image
    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using AIF (D39D2) XP® Rabbit mAb.
    Organelle Localization IF Antibody Sampler Kit: Image 24 Expand Image
    Immunohistochemical analysis of human ovarian serous cystadenoma using LAMP1 (D2D11) XP® Rabbit mAb.
    Organelle Localization IF Antibody Sampler Kit: Image 25 Expand Image
    Flow cytometric analysis of Jurkat cells using RCAS1 (D2B6N) XP® Rabbit mAb (blue) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red).
    Organelle Localization IF Antibody Sampler Kit: Image 26 Expand Image
    Confocal immunofluorescent analysis of NIH/3T3 cells using PDI (C81H6) Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
    Organelle Localization IF Antibody Sampler Kit: Image 27 Expand Image
    Confocal immunofluorescent analysis of mouse striatum using AIF (D39D2) XP® Rabbit mAb #5318 (green), GFAP (GA5) Mouse mAb #3670 (red), and F4/80 (BM8.1) Rat mAb #71299 (blue).
    Organelle Localization IF Antibody Sampler Kit: Image 28 Expand Image
    Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using AIF (D39D2) XP® Rabbit mAb.
    Organelle Localization IF Antibody Sampler Kit: Image 29 Expand Image
    Confocal immunofluorescent analysis of HeLa cells using LAMP1 (D2D11) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
    Organelle Localization IF Antibody Sampler Kit: Image 30 Expand Image
    Confocal immunofluorescent analysis of mouse thalamus using AIF (D39D2) XP® Rabbit mAb #5318 (green), GFAP (GA5) Mouse mAb #3670 (red). Sections were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
    Organelle Localization IF Antibody Sampler Kit: Image 31 Expand Image
    Immunohistochemical analysis of paraffin-embedded human papillary carcinoma of the breast using AIF (D39D2) XP® Rabbit mAb.
    Organelle Localization IF Antibody Sampler Kit: Image 32 Expand Image
    Flow cytometric analysis of Jurkat cells using LAMP1 (D2D11) XP® Rabbit mAb (solid line) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed line). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
    Organelle Localization IF Antibody Sampler Kit: Image 33 Expand Image
    Confocal immunofluorescent analysis of C2C12 cells using AIF (D39D2) XP® Rabbit mAb #5318 (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
    Organelle Localization IF Antibody Sampler Kit: Image 34 Expand Image
    Immunohistochemical analysis of paraffin-embedded normal human testis using AIF (D39D2) XP® Rabbit mAb.
    Organelle Localization IF Antibody Sampler Kit: Image 35 Expand Image
    Confocal immunofluorescent analysis of HeLa cells using AIF (D39D2) XP Rabbit mAb (green), showing colocalization with mitochondria that have been labeled with MitoTracker® Red CMXRos (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
    Organelle Localization IF Antibody Sampler Kit: Image 36 Expand Image
    Immunohistochemical analysis of paraffin-embedded normal human spleen using AIF (D39D2) XP® Rabbit mAb.
    Organelle Localization IF Antibody Sampler Kit: Image 37 Expand Image
    Immunohistochemical analysis of paraffin-embedded normal human skeletal muscle using AIF (D39D2) XP® Rabbit mAb.
    Organelle Localization IF Antibody Sampler Kit: Image 38 Expand Image
    Immunohistochemical analysis of paraffin-embedded normal human liver using AIF (D39D2) XP® Rabbit mAb.
    Organelle Localization IF Antibody Sampler Kit: Image 39 Expand Image
    Immunohistochemical analysis of paraffin-embedded normal human kidney using AIF (D39D2) XP® Rabbit mAb.
    Organelle Localization IF Antibody Sampler Kit: Image 40 Expand Image
    Immunohistochemical analysis of paraffin-embedded normal human breast using AIF (D39D2) XP® Rabbit mAb.
    Organelle Localization IF Antibody Sampler Kit: Image 41 Expand Image
    Immunohistochemical analysis of paraffin-embedded A20 syngeneic tumor using AIF (D39D2) XP® Rabbit mAb.
    Organelle Localization IF Antibody Sampler Kit: Image 42 Expand Image
    Immunohistochemical analysis of paraffin-embedded LL/2 syngeneic tumor using AIF (D39D2) XP® Rabbit mAb.
    Organelle Localization IF Antibody Sampler Kit: Image 43 Expand Image
    Immunohistochemical analysis of paraffin-embedded mouse testis using AIF (D39D2) XP® Rabbit mAb.
    Organelle Localization IF Antibody Sampler Kit: Image 44 Expand Image
    Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using AIF (D39D2) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).