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8653
Organelle Localization IF Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Organelle Localization IF Antibody Sampler Kit #8653

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Organelle Localization IF Antibody Sampler Kit: Image 1

Confocal immunofluorescent analysis of MCF7 cells, untreated (upper) or treated with Brefeldin A #9972 (5 μg/ml, 1 hr; lower), using RCAS1 (D2B6N) XP® Rabbit mAb #12290 (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Organelle Localization IF Antibody Sampler Kit: Image 2

Confocal immunofluorescent analysis of HeLa cells using EEA1 (C45B10) Rabbit mAb #3288 (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Organelle Localization IF Antibody Sampler Kit: Image 3

Confocal immunofluorescent analysis of NIH/3T3 cells using PDI (C81H6) Rabbit mAb #3501 (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Organelle Localization IF Antibody Sampler Kit: Image 4

Confocal immunofluorescent analysis of HeLa cells using AIF (D39D2) XP® Rabbit mAb #5318 (green), showing colocalization with mitochondria that were labeled with MitoTracker® Red CMXRos #9082 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Organelle Localization IF Antibody Sampler Kit: Image 5

Confocal immunofluorescent analysis of HeLa cells using LAMP1 (D2D11) XP® Rabbit mAb #9091 (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Organelle Localization IF Antibody Sampler Kit: Image 6

Confocal immunofluorescent analysis of HeLa cells using AIF (D39D2) XP ™ Rabbit mAb (green), showing colocalization with mitochondria that have been labeled with MitoTracker® Red CMXRos (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Organelle Localization IF Antibody Sampler Kit: Image 7

Confocal immunofluorescent analysis of HeLa cells using EEA1 (C45B10) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

Organelle Localization IF Antibody Sampler Kit: Image 8

Flow cytometric analysis of Jurkat cells using LAMP1 (D2D11) XP® Rabbit mAb (solid line) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed line). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Organelle Localization IF Antibody Sampler Kit: Image 9

Confocal immunofluorescent analysis of NIH/3T3 cells using PDI (C81H6) Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Organelle Localization IF Antibody Sampler Kit: Image 10

Flow cytometric analysis of Jurkat cells using RCAS1 (D2B6N) XP® Rabbit mAb (blue) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red).

Organelle Localization IF Antibody Sampler Kit: Image 11

Confocal immunofluorescent analysis of C2C12 cells using AIF (D39D2) XP® Rabbit mAb #5318 (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Organelle Localization IF Antibody Sampler Kit: Image 12

Western blot analysis of extracts from various cell lines using EEA1 (C45B10) Rabbit mAb.

Organelle Localization IF Antibody Sampler Kit: Image 13

Confocal immunofluorescent analysis of HeLa cells using LAMP1 (D2D11) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Organelle Localization IF Antibody Sampler Kit: Image 14

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using PDI (C81H6) Rabbit mAb.

Organelle Localization IF Antibody Sampler Kit: Image 15

Confocal immunofluorescent analysis of MCF7 cells, untreated (left) or treated with Brefeldin A #9972 (5 μg/ml, 1 hr; right), using RCAS1 (D2B6N) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Organelle Localization IF Antibody Sampler Kit: Image 16

Confocal analysis of mouse small intestine using AIF (D39D2) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Organelle Localization IF Antibody Sampler Kit: Image 17

Immunohistochemical analysis of normal human kidney using LAMP1 (D2D11) XP(R) Rabbit mAb.

Organelle Localization IF Antibody Sampler Kit: Image 18

Immunohistochemical analysis of paraffin-embedded human lymphoma using PDI (C81H6) Rabbit mAb.

Organelle Localization IF Antibody Sampler Kit: Image 19

Immunoprecipitation of RCAS1 from HCT 116 extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or RCAS1 (D2B6N) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using RCAS1 (D2B6N) XP® Rabbit mAb.

Organelle Localization IF Antibody Sampler Kit: Image 20

Western blot analysis of extracts from Jurkat, C2C12, and C6 cells using AIF (D39D2) XP® Rabbit mAb.

Organelle Localization IF Antibody Sampler Kit: Image 21

Immunohistochemical analysis of human lung carcinoma using LAMP1 (D2D11) XP(R) Rabbit mAb.

Organelle Localization IF Antibody Sampler Kit: Image 22

Immunohistochemical analysis of paraffin-embedded mouse spleen using PDI (C81H6) Rabbit mAb.

Organelle Localization IF Antibody Sampler Kit: Image 23

Western blot analysis of extracts from various cell lines using RCAS1 (D2B6N) XP® Rabbit mAb.

Organelle Localization IF Antibody Sampler Kit: Image 24

Immunohistochemical analysis of human ovarian serous cystadenoma using LAMP1 (D2D11) XP(R) Rabbit mAb.

Organelle Localization IF Antibody Sampler Kit: Image 25

Western blot analysis of extracts from various cell types using PDI (C81H6) Rabbit mAb.

Organelle Localization IF Antibody Sampler Kit: Image 26

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human RCAS1 (hRCAS1-Myc/DDK; +), using RCAS1 (D2B6N) XP® Rabbit mAb.

Organelle Localization IF Antibody Sampler Kit: Image 27

Western blot analysis of extracts from various cell lines using LAMP1 (D2D11) XP® Rabbit mAb.

To Purchase # 8653T
Product # Size Price
8653T
1 Kit  (5 x 20 µl) $ 391

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
AIF (D39D2) XP® Rabbit mAb 5318 20 µl
  • WB
  • IP
  • IF
H Mk M R 67 Rabbit IgG
EEA1 (C45B10) Rabbit mAb 3288 20 µl
  • WB
  • IP
  • IF
H M R 170 Rabbit IgG
LAMP1 (D2D11) XP® Rabbit mAb 9091 20 µl
  • WB
  • IP
  • IHC
  • IF
  • F
H Mk 42 (non-glycosylated), 90-120 (glycosylated) Rabbit IgG
PDI (C81H6) Rabbit mAb 3501 20 µl
  • WB
  • IHC
  • IF
H Mk M R 57 Rabbit 
RCAS1 (D2B6N) XP® Rabbit mAb 12290 20 µl
  • WB
  • IP
  • IF
  • F
H M R 32 Rabbit IgG

Product Description

The Organelle Localization IF Antibody Sampler Kit provides an economical means for identification of cellular organelles by fluorescence immunocytochemistry (IF-IC). This kit includes enough primary antibody to perform at least twenty IF-IC tests or two western blots with each antibody.

Specificity / Sensitivity

Each antibody in the Organelle Localization IF Antibody Sampler Kit recognizes only its specific target and does not cross-react with other family members. Each antibody has been validated for IF-IC and stains the organelles indicated above. Expression of these proteins may vary in different cells and tissues. Please see www.cellsignal.com for additional specificity/sensitivity information for individual kit components.

Source / Purification

Rabbit monoclonal antibodies are produced by immunizing animals with a recombinant protein fragment of human LAMP1 protein, or with a synthetic peptide corresponding to residues surrounding Ala520 of human AIF protein, residues surrounding Ser70 of human EEA1 protein, the sequence of human PDI protein, or residues surrounding Gly147 of human RCAS1 protein.

Background

Knowledge of the subcellular location of a protein may reveal its potential role in a variety of cellular processes. One can confirm the subcellular location of a marker that colocalizes with one of the organelle-specific antibodies in this kit. While these antibodies serve as powerful tools for immunofluorescence, they may also be used as western blot controls for fractionated cell lysates.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
DRAQ5 is a registered trademark of Biostatus Limited.
MitoTracker is a registered trademark of Molecular Probes, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.