WB
H
Endogenous
31, 33
Rabbit IgG
#P32243
5015
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Species predicted to react based on 100% sequence homology
The antigen sequence used to produce this antibody shares
100% sequence homology with the species listed here, but
reactivity has not been tested or confirmed to work by CST.
Use of this product with these species is not covered under
our
Product Performance Guarantee.
Mouse, Rat, Monkey, Chicken, Bovine, Horse, Guinea Pig
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn251 of human OTX2 protein.
Background
Orthodenticle homeobox 2 (OTX2) belongs to the bicoid subfamily of paired-box, homeodomain-containing transcription factors. OTX2 is a critically important neuronal transcription factor that functions to regulate the expression of cell cycle genes controlling proliferation and differentiation of neural progenitor cells (1-3). In addition to its neuronal development functions, it has been reported that OTX2 can function in a non-cell autonomous manner to promote survival of damaged retinal ganglion cells (4). OTX2 has also been shown to influence the susceptibility of post-mitotic neurons to toxic insult or physiological stress (3). Notably, aberrant expression of OTX2 has been strongly linked with neuronal tumor development. For example, research studies have found OTX2 is overexpressed in many medulloblastoma cell lines, and both overexpression and gene amplification were reported in a subset of primary medulloblastomas (5). In vitro studies support these observations, as targeted alterations in OTX2 expression directly affected both proliferation and senescence of medulloblastoma cell lines (6,7).
- Simeone, A. et al. (1993) EMBO J 12, 2735-47.
- Omodei, D. et al. (2008) Development 135, 3459-70.
- Chung, C.Y. et al. (2010) Brain 133, 2022-31.
- Torero Ibad, R. et al. (2011) J Neurosci 31, 5495-503.
- Adamson, D.C. et al. (2010) Cancer Res 70, 181-91.
- Bunt, J. et al. (2011) Int J Cancer 131, E21-32.
- Bunt, J. et al. (2010) Mol Cancer Res 8, 1344-57.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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