Render Target: STATIC
Render Timestamp: 2024-12-02T12:04:28.225Z
Commit: cd2fae6ca3f811b1ddb1df24ac291ed56d5d501b
XML generation date: 2024-05-29 14:14:06.400
Product last modified at: 2024-11-20T20:45:07.817Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

p16 INK4A (BC42) Mouse mAb #68410

Filter:
  • IHC

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Mouse IgG1 kappa
    Application Key:
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    IHC Leica Bond 1:50 - 1:200
    Immunohistochemistry (Paraffin) 1:50

    Storage

    Supplied in a Tris-based buffer with ~1% BSA and less than 0.1% sodium azide. Stable for 6 months when stored at 4°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    p16 INK4A (BC42) Mouse mAb recognizes endogenous levels of total p16 INK4A protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a prokaryotic recombinant protein corresponding to the p16 INK4A region of the p16 molecule.

    Background

    Members of the INK4 family of cyclin-dependent kinase inhibitors include p16 INK4A, p15 INK4B, p18 INK4C, and p19 INK4D. The INK4 family members inhibit cyclin-dependent kinases 4 and 6 (CDK4 and CDK6), causing cell cycle arrest in G1 phase. The INK4A-ARF-INK4B locus on chromosome 9p21, frequently lost in human cancer, encodes the INK4 family members p16 INK5A and p15 INK4B, as well as the unrelated protein, ARF (1).

    p16 INK4A expression, typically repressed in the absence of stress, is thought to drive cells into senescence, and p16 INK4A expression is a commonly used marker of senescent cells (2,3). p16 INK4A protein expression is often altered in human cancer, and high expression is currently used as a predictive biomarker in cervical cancer (3-5).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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