WB, IP
H R
Endogenous
22
Rabbit
#P13498
1535
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human p22phox protein. Antibodies are purified by peptide affinity chromatography.
Background
The p22phox protein is a critical component of the membrane-bound NADPH Oxidase of phagocytes that generate superoxide anion and alter the redox states in cells. p22phox associates with the catalytic subunit gp91phox (NOX2), NOX1, NOX3, or NOX4 to form the active NADPH Oxidase. The enzymatic complex includes cytosolic subunits p40phox, p47phox, p67phox, and Rac2 that are recruited to the membrane-bound subunits, subsequently generating superoxide anion as the final product, serving as a microbial killing system (1,2). Multiple phosphorylation sites in each subunit of these oxidases potentiate its activation, including the phosphorylation of p22phox at Thr147 epitope facing the cytoplasmic space (3-5). NADPH Oxidase is associated with several diseases, including hyperglycemia, cancer, inflammation and vascular cases, as well as neurodegenerative diseases (6), including its activation by SOD1 mutants in Amyotrophic Lateral Sclerosis (7), and in Alzheimer's disease where NADPH Oxidase activation exacerbates the effects of fibrillary tangles and β-amyloid plaques, causing neuronal death (8,9).
- Nauseef, W.M. (2019) Curr Opin Immunol 60, 130-140.
- Brandes, R.P. et al. (2014) Free Radic Biol Med 76, 208-26.
- Belambri, S.A. et al. (2018) Eur J Clin Invest 48 Suppl 2, e12951.
- Lewis, E.M. et al. (2010) J Biol Chem 285, 2959-67.
- Boillée, S. and Cleveland, D.W. (2008) J Clin Invest 118, 474-8.
- Rastogi, R. et al. (2016) Front Cell Neurosci 10, 301.
- Shiose, A. and Sumimoto, H. (2000) J Biol Chem 275, 13793-801.
- Bianca, V.D. et al. (1999) J Biol Chem 274, 15493-9.
- Chay, K.O. et al. (2017) Chonnam Med J 53, 196-202.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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