Revision 1
#70659
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W
Reactivity:
H
Sensitivity:
Endogenous
MW (kDa):
62
Source/Isotype:
Rabbit IgG
UniProt ID:
#Q99571
Entrez-Gene Id:
5025
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity/Sensitivity
Source / Purification
Background
P2X purinoceptor 4 (P2X4) trimers are expressed at the cell surface where they act as ligand-gated ion channels for monovalent and divalent cations (3). P2X4 receptors contribute to regulation of synaptic strength through participation in the formation of long-term potentiation (4,5). Research studies indicate that P2X4 receptor expression may play a role in influencing alcohol-drinking behavior and conferring protection to cardiac myocytes during heart failure (6,7). Additional studies show that microglial P2X4 receptors are upregulated during nerve-injury associated neuropathic pain (8).
Background References
- North, R.A. (2002) Physiol Rev 82, 1013-67.
- Valera, S. et al. (1994) Nature 371, 516-9.
- Abbracchio, M.P. et al. (2009) Trends Neurosci 32, 19-29.
- Fujii, S. (2004) J Pharmacol Sci 94, 103-6.
- Sim, J.A. et al. (2006) J Neurosci 26, 9006-9.
- Franklin, K.M. et al. (2014) Front Neurosci 8, 176.
- Yang, T. et al. (2014) Circ Heart Fail 7, 510-8.
- Tsuda, M. et al. (2013) Front Cell Neurosci 7, 191.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting
Cross-Reactivity Key
H: Human
Trademarks and Patents
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
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