Western blot analysis of purified recombinant full-length p38 MAPK GST-fusion proteins, using p38β MAPK (C28C2) Rabbit mAb (upper), or a p38 MAPK pan antibody (lower).
Western blot analysis of purified recombinant full-length p38 MAPK GST fusion protein, using p38 MAP kinase pan antibody (upper) or p38γ MAPK Antibody (lower).
Western blot analysis of purified recombinant full-length p38 MAPK GST fusion proteins, using p38 MAPK pan antibody (upper), or p38δ MAPK (10A8) Rabbit mAb (lower).
Flow cytometric analysis of Jurkat cells, untreated (blue) or anisomycin-treated (green), using Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAb compared to a nonspecific negative control antibody (red).
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
Western blot analysis of extracts from HeLa, NIH/3T3, PC12 and COS cells, using p38α MAPK Antibody.
Westen blot analysis of extracts from HUVEC and COS cells using p38β MAPK (C28C2) Rabbit mAb.
Western blot analysis of extracts from HUVEC and U937 cells, using p38γ MAPK Antibody.
Western blot analysis of extracts from 293, NBT-II and PC12 cells, using p38δ MAPK (10A8) Rabbit mAb.
Confocal immunofluorescent analysis of COS cells, untreated (left) or anisomycin-treated (right) using Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).
Western blot analysis of extracts from Xenopus oocytes overexpressing Myc-tagged p38 isoforms, using p38α MAPK Antibody (upper) and Myc-Tag Antibody (lower) to demonstrate the isoform specificity of p38α MAPK Antibody. (Provided by Dr. Eusebio Perdiguero and Dr. Angel Nebreda, European Molecular Biology Laboratory, Germany.)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse colon using Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded 293T cell pellets, untreated (left) or UV-treated (right), using Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAb.
Western blot analysis of extracts from COS and 293 cells, untreated or UV-treated, using Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAb (upper) or p38 MAPK Antibody #9212 (lower).
|p38α MAPK Antibody 9218||20 µl||
||H M R Mk||40||Rabbit|
|p38β MAPK (C28C2) Rabbit mAb 2339||20 µl||
||H Mk||43||Rabbit IgG|
|p38γ MAPK Antibody 2307||20 µl||
||H M R Mk||46||Rabbit|
|p38δ MAPK (10A8) Rabbit mAb 2308||20 µl||
||H R||43||Rabbit IgG|
|Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAb 4511||20 µl||
||H M R Mk Mi Pg Sc||43||Rabbit IgG|
|Anti-rabbit IgG, HRP-linked Antibody 7074||100 µl||
The p38 MAPK Isoform Activation Antibody Sampler Kit provides an economical means to evaluate the activation status of individual isoforms of p38 MAPK through immunoprecipitation of the phosphorylated p38 MAPK followed by western blot using isoform specific antibodies. The kit includes enough primary and secondary antibodies to perform two IP/western blot experiments.
Each isoform-specific antibody in the p38 MAPK Isoform Activation Antibody Sampler Kit recognizes the appropriate isoform of p38 MAPK. The Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAb recognizes all four isoforms of p38 MAPK when phosphorylated at the specified sites.
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to carboxy-terminal residues of human p38α MAPK or human p38γ MAPK. Antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the carboxy terminus of p38β MAPK or p38δ MAPK and surrounding Thr180/Tyr182 of human p38 MAPK.
p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAPK, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8). SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP-binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10).
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