WB
M R
Endogenous
50
Rabbit
#P22777
18787
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly196 of murine PAI-1 protein. Antibodies are purified by peptide affinity chromatography.
Background
PAI-1 is a secreted protein that belongs to the serine proteinase inhibitor (serpin) superfamily. It inhibits urokinase and tissue plasminogen activators (uPA and tPA) and thus, reduces the conversion of inactive plasminogen to plasmin (1). PAI-1 regulates fibrinolysis and plays an important role in vessel patency and tissue remodeling. Secreted PAI-1 interacts with the extracellular matrix (ECM) component vitronectin, thereby modulating cell-ECM interactions (2,3). PAI-1 is expressed in a variety of tissues with higher expression in liver, vascular endothelial cells, platelets, macrophages, and adipose tissue (1). Increased levels of PAI-1 are associated with deep vein thrombosis (4). Defects in PAI-1 cause plasminogen activator inhibitor-1 deficiency (PAI-1D), which is characterized by increased bleeding after injury or surgery (5). Research studies have shown that high levels of PAI-1 are associated with obesity, aging, insulin resistance, and type 2 diabetes (6-8). PAI-1 is transcriptionally regulated by TGF-β and mediates TGF-β-induced inhibition of cell migration and invasion in cancer cells (9). Studies have also shown PAI-1 to be involved in fibrosis (10).
- Pannekoek, H. et al. (1986) EMBO J 5, 2539-44.
- Sigurdardottir, O. and Wiman, B. (1994) Biochim Biophys Acta 1208, 104-10.
- Konstantinides, S. et al. (2001) Circulation 103, 576-83.
- Baldwin, J.F. et al. (2012) J Vasc Surg 56, 1089-97.
- Fay, W.P. et al. (1997) Blood 90, 204-8.
- Pannacciulli, N. et al. (2002) Obes Res 10, 717-25.
- Juhan-Vague, I. et al. (1991) Diabetologia 34, 457-62.
- Hashimoto, Y. et al. (1987) Thromb Res 46, 625-33.
- Humbert, L. and Lebrun, J.J. (2012) Cell Signal 25, 490-500.
- Zhang, L.P. et al. (1999) J Hepatol 31, 703-11.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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