WB, IHC-P
H M R
Endogenous
140, 90
Rabbit IgG
#Q8WX93
23022
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunohistochemistry (Paraffin) | 1:400 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro1043 of human palladin protein.
Background
The actin-associated scaffold protein, palladin, is associated with multiple actin-rich structures, and plays a role in cellular migration and invasion (1). Palladin regulates the formation of invasive structures called podosomes and invadopodia (1-3). Alternative splicing results in at least three palladin isoforms, including the widely expressed 140 kDa and 90 kDa isoforms (2). Palladin has been shown to promote invasion in basal-like breast carcinoma models (4). Palladin is also upregulated in injured kidneys, and promotes migration of kidney cells to facilitate repair (5). In differentiating muscle cells, palladin regulates migration and myogenesis (6). Several research studies have focused on palladin expression and function in cancer-associated fibroblasts (CAFs). Researchers have shown that expression of palladin in stromal fibroblasts of pancreatic ductal adenocarcinoma is an indication of the effectiveness of chemotherapy (7). The cancer associated transcription factor Twist1 may require palladin and collagen alpha1 for its metastatic effect in fibroblasts (8).
- Goicoechea, S.M. et al. (2008) Eur J Cell Biol 87, 517-25.
- Najm, P. and El-Sibai, M. (2014) Cell Adh Migr 8, 29-35.
- Goicoechea, S.M. et al. (2014) Oncogene 33, 1265-73.
- von Nandelstadh, P. et al. (2014) Mol Biol Cell 25, 2556-70.
- Chang, E.H. et al. (2015) Sci Rep 5, 7695.
- Nguyen, N.U. and Wang, H.V. (2015) PLoS One 10, e0124762.
- Sato, D. et al. (2016) PLoS One 11, e0152523.
- García-Palmero, I. et al. (2016) Oncogene , .
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IHC-P: Immunohistochemistry (Paraffin)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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