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17171
Pan-Keratin (C11) Mouse mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody
R
Recombinant

Pan-Keratin (C11) Mouse mAb (BSA and Azide Free) #17171

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  1. WB
  2. IHC
  3. IF
  4. F
Western blot analysis of extracts from various cell lines, using Pan-Keratin (C11) Mouse mAb. Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Pan-Keratin (C11) Mouse mAb. Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using Pan-Keratin (C11) Mouse mAb. Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using Pan-Keratin (C11) mouse mAb. Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma using Pan-Keratin (C11) mouse mAb. Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded human ductal breast carcinoma using Pan-Keratin (C11) mouse mAb. Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded human transitional epithelial carcinoma (bladder) using Pan-Keratin (C11) Mouse mAb. Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Pan-Keratin (C11) Mouse mAb. Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded H358 xenograft using Pan-Keratin (C11) Mouse mAb. Data were generated using the standard formulation of this product.
Confocal immunofluorescent analysis of HeLa cells using Pan-Keratin (C11) Mouse mAb (green) and DRAQ5® #4084 (fluorescent DNA dye). Data were generated using the standard formulation of this product.
Confocal immunofluorescent analysis of HeLa cells using Pan-Keratin (C11) Mouse mAb (green), DyLight 554 Phalloidin #13054 (red), and DRAQ5 (fluorescent DNA dye). Data were generated using the standard formulation of this product.
Flow cytometric analysis of A-431 cells using Pan-Keratin (C11) Mouse mAb (solid line) versus a concentration-matched Mouse (G3A1) mAb IgG1 Isotype Control #5415 (dashed line). Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4408 was used as a secondary antibody. Data were generated using the standard formulation of this product.

Supporting Data

REACTIVITY H M R Mk
SENSITIVITY Endogenous
MW (kDa) 46-58
Source/Isotype Mouse IgG1

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • C&R-CUT&RUN
  • C&T-CUT&Tag
  • DB-Dot Blot
  • eCLIP-eCLIP
  • IF-Immunofluorescence
  • F-Flow Cytometry

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • GP-Guinea Pig
  • Rab-Rabbit
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #4545. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free.

For standard formulation of this product see product #4545.

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

Pan-Keratin (C11) Mouse mAb (BSA and Azide Free) detects endogenous levels of total keratin 4, 5, 6, 8, 10, 13 and 18. The antibody does not cross-react with other keratins.

Species Reactivity:

Human, Mouse, Rat, Monkey

Source / Purification

Monoclonal antibody (isotype: IgG1) is produced by immunizing a BALB/c mouse with a cytoskeleton preparation from A-431 cells.

Background

Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins K9-K28) and a basic keratin (or type II keratin, keratins K1-K8 and K71-K80) assemble to form filaments. Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as research and clinical biomarkers (1,2).

Dysregulation/mutations in keratin genes can lead to a variety of disorders affecting the skin, hair, nails, and other epithelial tissues (3). While expression of keratins can be variable, immunohistochemical staining of keratins is widely used to help in the identification and classification of epithelial tumors, and may also provide prognostic information.

Keratins 8 and 18 (K8/K18) are expressed in simple epithelia of normal tissue, as well as in adenocarcinomas of the breast, lung, ovary, and gastrointestinal tract. Keratin 17 is expressed in basal keratinocytes of stratified epithelia, hair follicles, and sebaceous glands. Onset of keratin 17 expression coincides with the definition of major epithelial lineages during skin development (4). Keratin 14 (K14) is expressed in basal cells of stratified epithelia, and in basal-like subtypes of breast cancer and squamous cell carcinomas. Keratin 19 (K19) is expressed in glandular epithelia, including the liver, gallbladder, and pancreas, as well as in adenocarcinomas of the breast, thyroid, and bile duct. Keratin 20 (K20) is expressed in gastrointestinal epithelium, urothelium, and Merkel cells in the skin, as well as in colorectal carcinomas and some urothelial carcinomas. Keratin 5/6 (K5/6) is expressed in basal cells of stratified epithelia, including the skin, prostate, and breast, as well as in basal-like breast cancers, squamous cell carcinomas, and some lung carcinomas. Keratin 7 (K7) is expressed in glandular epithelia, such as those in the lung, breast, and female reproductive tract, as well as in adenocarcinomas of the lung, breast, and ovary (5,6).

Keratins, particularly K8, K18, and K19, serve as biomarkers for identification of circulating tumor cells (CTCs) (5).

Post-translational modifications, including phosphorylation, acetylation, ubiquitylation, sumoylation, glycosylation, and transamidation, have been shown to affect the functions of keratins in normal and disease states (6). Understanding the molecular mechanisms underlying these PTMs may provide insights into cancer pathogenesis.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not for Use in Diagnostic Procedures.
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