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8648
Parkinson's Research Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Parkinson's Research Antibody Sampler Kit #8648

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Parkinson's Research Antibody Sampler Kit: Image 1

Confocal immunofluorescent analysis of MEF wild-type (left) or MEF DJ-1 (-/-) (right) cells using DJ-1 (D29E5) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). (MEF wild-type and MEF DJ-1 (-/-) cells were kindly provided by Dr. Philipp Kahle, University of Tübingen, Germany).

Parkinson's Research Antibody Sampler Kit: Image 2

Immunoprecipitation of LRRK2 from mouse brain extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is LRRK2 (D18E12) Rabbit mAb. Western blot analysis was performed using LRRK2 (D18E12) Rabbit mAb. Anti-rabbit, HRP-linked Antibody #7074 was used as a secondary antibody.

Parkinson's Research Antibody Sampler Kit: Image 3

Western blot analysis of extracts from PC12 cells, fetal rat brain and mouse brain, using Parkin (Prk8) Mouse mAb.

Parkinson's Research Antibody Sampler Kit: Image 4

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a cDNA construct expressing full-length human PINK1 (hPINK1, +) using PINK1 (D8G3) Rabbit mAb.

Parkinson's Research Antibody Sampler Kit: Image 5

Confocal immunofluorescent analysis of normal rat cerebellum, hippocampus and striatum using α-Synuclein (D37A6) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Parkinson's Research Antibody Sampler Kit: Image 6

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Parkinson's Research Antibody Sampler Kit: Image 7

Western blot analysis of extracts from MEF wild-type, MEF DJ-1 (-/-), HeLa, and C6 cells using DJ-1 (D29E5) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). (MEF wild-type and MEF DJ-1 (-/-) cells were kindly provided by Dr. Philipp Kahle, University of Tübingen, Germany).

Parkinson's Research Antibody Sampler Kit: Image 8

Western blot analysis of extracts from U-87 MG and A172 cells, and mouse brain using LRRK2 (D18E12) Rabbit mAb. 

Parkinson's Research Antibody Sampler Kit: Image 9

Western blot analysis of extracts from HeLa cells, untreated (-) or treated with CCCP (10 μM, 24 hr; +), using PINK1 (D8G3) Rabbit mAb.

Parkinson's Research Antibody Sampler Kit: Image 10

Immunohistochemical analysis of paraffin-embedded mouse brain using α-Synuclein (D37A6) XP® Rabbit mAb.

Parkinson's Research Antibody Sampler Kit: Image 11

Western blot analysis of extracts from mouse and rat brain using α-Synuclein (D37A6) XP® Rabbit mAb.

To Purchase # 8648T
Product # Size Price
8648T
1 Kit  (5 x 20 µl) $ 391

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
DJ-1 (D29E5) XP® Rabbit mAb 5933 20 µl
  • WB
  • IP
  • IF
H M R Hm Mk 22 Rabbit IgG
LRRK2 (D18E12) Rabbit mAb 13046 20 µl
  • WB
  • IP
H M R 290 Rabbit IgG
Parkin (Prk8) Mouse mAb 4211 20 µl
  • WB
  • IP
H M R 50 Mouse IgG2b
PINK1 (D8G3) Rabbit mAb 6946 20 µl
  • WB
  • IP
H 60, 50 Rabbit IgG
α-Synuclein (D37A6) XP® Rabbit mAb 4179 20 µl
  • WB
  • IP
  • IHC
  • IF
M R 18 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 
Anti-mouse IgG, HRP-linked Antibody 7076 100 µl
  • WB
Horse 

Product Description

The Parkinson's Research Antibody Sampler Kit provides an economical means of detecting target proteins related to Parkinson's disease. The kit contains enough primary and secondary antibody to perform two western blots per primary.

Specificity / Sensitivity

DJ-1 (D29E5) XP® Rabbit mAb, LRRK2 (D18E12) Rabbit mAb, Parkin (Prk8) Mouse mAb, and PINK1 (D8G3) Rabbit mAb recognize endogenous levels of respective target proteins. α-Synuclein (D37A6) XP® Rabbit mAb recognizes endogenous levels of the α isoform of synuclein protein.

Source / Purification

Monoclonal antibodies are produced by immuninzing animals with a recombinant protein specific to the carboxy terminus of Parkin protein, a synthetic peptide corresponding to residues surrounding Lys148 of human DJ-1 protein, a synthetic peptide corresponding to residues surrounding Pro2080 of human LRRK2 protein, a synthetic peptide corresponding to residues surrounding Pro140 of human PINK1 protein, or a synthetic peptide corresponding to residues surrounding Glu105 of mouse α-synuclein protein.

Background

Parkinson’s disease (PD), the second most common neurodegenerative disease after Alzheimer’s, is a progressive movement disorder characterized by rigidity, tremors, and postural instability. The pathological hallmark of PD is progressive loss of dopaminergic neurons in the substantia nigra of the ventral midbrain and the presence of intracellular Lewy bodies in surviving neurons of the brain stem (1). Research studies have shown that various genes and loci (α-synuclein/PARK1 and 4, parkin/PARK2, UCH-L1/PARK5, PINK1/PARK6, DJ-1/PARK7, LRRK2/PARK8, synphilin-1, and NR4A2) are genetically linked to PD (2).

α-Synuclein, a 140 amino acid protein expressed abundantly in the brain, is a major component of aggregates found in Lewy bodies (3). Parkin is involved in protein degradation through the ubiquitin-proteasome pathway, and investigators have shown that mutations in Parkin cause early onset of PD (4). In the case of autosomal recessive juvenile Parkinsonism (AR-JP), deletions have been found on chromosome 6 in the Parkin gene (5). PTEN induced putative kinase 1 (PINK1) is a mitochondrial serine/threonine kinase involved in the normal function and integrity of mitochondria, as well as a reduction of cytochrome c release from mitochondria (6-8). PINK1 phosphorylates Parkin and promotes its translocation to mitochondria (7). Mutations of PINK1 are associated with loss of protective function, mitrochondrial dysfunction, aggregation of α-synuclein, and proteasome dysfunction (6,8). DJ-1 is involved in multiple cellular functions; it has been shown to cooperate with Ras to increase cell transformation, to regulate transcription of the androgen receptor, and may function as an indicator of oxidative stress, while loss-of-function mutations in DJ-1 cause early onset of PD (9-12). Dopamine D2 receptor-mediated functions are greatly impaired in DJ-1 (-/-) mice, resulting in reduced long-term depression (13). Leucine-rich repeat kinase 2 (LRRK2) contains amino-terminal leucine-rich repeats (LRR), a Ras-like small GTP binding protein-like (ROC) domain, an MLK protein kinase domain, and a carboxy-terminal WD40-repeat. At least 20 LRRK2 mutations have been linked to PD (14). The most prevalent mutation, G2019S, causes increased LRRK2 kinase activity, leading to progressive neurite loss and decreased neuronal survival (15).

  1. Fahn, S. (2003) Ann N Y Acad Sci 991, 1-14.
  2. Moore, D.J. et al. (2005) Annu Rev Neurosci 28, 57-87.
  3. Goldberg, M.S. and Lansbury Jr., P.T. (2000) Nat. Cell Biol. 2, 115-119.
  4. Borrelli, E. (2005) Neuron 45, 479-81.
  5. Polymeropoulos, M.H. et al. (1997) Science 276, 2045-7.
  6. Liu, W. et al. (2009) PLoS One 4, e4597.
  7. Kim, Y. et al. (2008) Biochem Biophys Res Commun 377, 975-80.
  8. Petit, A. et al. (2005) J Biol Chem 280, 34025-32.
  9. Bonifati, V. et al. (2003) Science 299, 256-9.
  10. Nagakubo, D. et al. (1997) Biochem. Biophys. Res. Commun. 231, 509-13.
  11. Takahashi, K. et al. (2001) J. Biol. Chem. 276, 37556-63.
  12. Mitsumoto, A. and Nakagawa, Y. (2001) Free Radic. Res. 35, 885-93.
  13. Goldberg, M.S. et al. (2005) Neuron 45, 489-96.
  14. Mata, I.F. et al. (2006) Trends Neurosci. 29, 286-293.
  15. MacLeod, D. et al. (2006) Neuron 52, 587-593.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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