Revision 8

#26768

Store at -20C

Parthanatos Antibody Sampler Kit

1 Kit

(4 x 20 microliters)

CST Logo
Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Product Includes Product # Quantity Mol. Wt Isotype/Source
PARP (46D11) Rabbit Monoclonal Antibody953220 µl116, 89 kDaRabbit
AIF (D39D2) Rabbit Monoclonal Antibody531820 µl67 kDaRabbit IgG
MIF (E8S8H) Rabbit Monoclonal Antibody7503820 µl12 kDaRabbit IgG
Anti-rabbit IgG, HRP-linked Antibody7074100 µlGoat
Poly/Mono-ADP Ribose (D9P7Z) Rabbit Monoclonal Antibody8919020 µlRabbit IgG

Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.

Description

The Parthanatos Antibody Sampler Kit provides an economical means of detecting the activation of parthanatos. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibodies.

Background

Parthanatos is a form of regulated cell death that follows a multistep cascade and is triggered by the accumulation of poly (ADP-ribose) (PAR). When PAR polymerase-1 (PARP-1) is overactivated under certain conditions, excessive PAR is produced and binds to apoptosis-inducing factor (AIF). As a result, AIF is released from the mitochondria and forms a complex with macrophage migration inhibitory factor (MIF). Subsequently, the AIF/MIF complex is translocated to the nucleus where MIF cleaves genomic DNA into large fragments, and cell death follows (1-3). Studies have found that parthanatos is involved in the pathogenesis of many diseases, particularly neurodegenerative disorders, such as Alzheimer's disease, Huntington's disease, Parkinson's disease, and amyotrophic lateral sclerosis (ALS) (4-7).

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 8

Cell Signaling Technology Logo
Western blot analysis of extracts from Jurkat, C2C12, and C6 cells using AIF (D39D2) XP® Rabbit mAb.
undefined Image 1: Parthanatos Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using AIF (D39D2) XP® Rabbit mAb.
undefined Image 2: Parthanatos Antibody Sampler Kit
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
undefined Image 3: Parthanatos Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 8

Cell Signaling Technology Logo
Western blot analysis of extracts from various cell lines using MIF (E8S8H) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). The absence of MIF protein in SNB-19 cells is consistent with mRNA expression profiles reported in public bioinformatic databases, confirming specificity of the antibody for MIF.
undefined Image 4: Parthanatos Antibody Sampler Kit
Western blot analysis of extracts from HeLa cells untreated (-), treated (+) with hydrogen peroxide (0.5 mM, 5 min), or treated with Parp inhibitor Talazoparib #80577 (100 nM, 3 hr) prior to hydrogen peroxide treatment (0.5 mM, 5 min), using Poly/Mono-ADP Ribose (D9P7Z) Rabbit mAb (upper) or B-Actin (13E5) Rabbit mAb #4970 (lower) and detected with Anti-rabbit IgG (H+L) (DyLight 680 Conjugate) #5366.
undefined Image 5: Parthanatos Antibody Sampler Kit
Flow cytometric analysis of HeLa cells treated with Talazoparib #80577 (100 nM, 3 hr) then hydrogen peroxide (500 mM, 5 min) (blue), or treated with hydrogen peroxide alone (500 mM, 5 min) (green), using Poly/Mono-ADP Ribose (D9P7Z) Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
undefined Image 6: Parthanatos Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 8

Cell Signaling Technology Logo
Western blot analysis of extracts from control HEK293 cells (lane 1) or PARP knockout HEK293 cells (lane 2) using PARP (46D11) Rabbit mAb #9532 (upper) or β-actin (13E5) Rabbit mAb #4970 (lower). The absence of signal in the PARP knockout HEK293 cells confirms the specificity of the antibody for PARP.
undefined Image 7: Parthanatos Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human papillary thyroid carcinoma using AIF (D39D2) XP® Rabbit mAb.
undefined Image 8: Parthanatos Antibody Sampler Kit
Confocal immunofluorescent analysis of mouse testis (left) and skeletal muscle (right) using MIF (E8S8H) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
undefined Image 9: Parthanatos Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 8

Cell Signaling Technology Logo
Confocal immunofluorescent analysis of HeLa cells, either untreated (left), treated with hydrogen peroxide (500 mM, 5 min; middle), or treated with PARP inhibitor Talazoparib #80577 (100 nmol/L, 3 hr) followed by hydrogen peroxide (500 mM, 5 min; right), using Poly/Mono-ADP Ribose (D9P7Z) Rabbit mAb (green), β-Actin (8H10D10) Mouse mAb #3700 (red), and DAPI #4083 (blue).
undefined Image 10: Parthanatos Antibody Sampler Kit
Western blot analysis of extracts from THP-1 cells, untreated or treated with TNF-α and cycloheximide as well as control extracts from SW620 and A20 cell lines, using PARP (46D11) Rabbit mAb.
undefined Image 11: Parthanatos Antibody Sampler Kit
Confocal immunofluorescent analysis of mouse brainstem using AIF (D39D2) XP® Rabbit mAb #5318 (green) and GFAP (GA5) Mouse mAb #3670 (red). Sections were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
undefined Image 12: Parthanatos Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 8

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using AIF (D39D2) XP® Rabbit mAb.
undefined Image 13: Parthanatos Antibody Sampler Kit
Confocal immunofluorescent analysis of U-87 MG cells (left, positive) or SNB-19 cells (right, negative) using MIF (E8S8H) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
undefined Image 14: Parthanatos Antibody Sampler Kit
Confocal immunofluorescent analysis of HeLa cells, either treated with hydrogen peroxide (500 mM, 5 min; left) or treated with hydrogen peroxide (500 mM, 5 min), then treated post fixation with recombinant human PARG (right), using Poly/Mono-ADP Ribose (D9P7Z) Rabbit mAb (green), β-Actin (8H10D10) Mouse mAb #3700 (red), and DAPI #4083 (blue).
undefined Image 15: Parthanatos Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 8

Cell Signaling Technology Logo
Confocal immunofluorescent analysis of mouse cerebellum using AIF (D39D2) XP® Rabbit mAb #5318 (green), GFAP (GA5) Mouse mAb #3670 (red), and F4/80 (BM8.1) Rat mAb #71299 (blue).
undefined Image 16: Parthanatos Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using AIF (D39D2) XP® Rabbit mAb.
undefined Image 17: Parthanatos Antibody Sampler Kit
Western blot analysis of extracts from C6 and NIH/3T3 cells that were serum starved overnight, untreated (-) or treated (+) with hydrogen peroxide (1,000 μM, 5 min), using Poly/Mono-ADP Ribose (D9P7Z) Rabbit mAb (upper) or B-Actin (13E5) Rabbit mAb # 4970.
undefined Image 18: Parthanatos Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 8

Cell Signaling Technology Logo
Confocal immunofluorescent analysis of mouse striatum using AIF (D39D2) XP® Rabbit mAb #5318 (green), GFAP (GA5) Mouse mAb #3670 (red), and F4/80 (BM8.1) Rat mAb #71299 (blue).
undefined Image 19: Parthanatos Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using AIF (D39D2) XP® Rabbit mAb.
undefined Image 20: Parthanatos Antibody Sampler Kit
Confocal immunofluorescent analysis of mouse thalamus using AIF (D39D2) XP® Rabbit mAb #5318 (green), GFAP (GA5) Mouse mAb #3670 (red). Sections were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
undefined Image 21: Parthanatos Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 8

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human papillary carcinoma of the breast using AIF (D39D2) XP® Rabbit mAb.
undefined Image 22: Parthanatos Antibody Sampler Kit
Confocal immunofluorescent analysis of C2C12 cells using AIF (D39D2) XP® Rabbit mAb #5318 (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
undefined Image 23: Parthanatos Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded normal human testis using AIF (D39D2) XP® Rabbit mAb.
undefined Image 24: Parthanatos Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 8

Cell Signaling Technology Logo
Confocal immunofluorescent analysis of HeLa cells using AIF (D39D2) XP ™ Rabbit mAb (green), showing colocalization with mitochondria that have been labeled with MitoTracker® Red CMXRos (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
undefined Image 25: Parthanatos Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded normal human spleen using AIF (D39D2) XP® Rabbit mAb.
undefined Image 26: Parthanatos Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded normal human skeletal muscle using AIF (D39D2) XP® Rabbit mAb.
undefined Image 27: Parthanatos Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 8

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded normal human liver using AIF (D39D2) XP® Rabbit mAb.
undefined Image 28: Parthanatos Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded normal human kidney using AIF (D39D2) XP® Rabbit mAb.
undefined Image 29: Parthanatos Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded normal human breast using AIF (D39D2) XP® Rabbit mAb.
undefined Image 30: Parthanatos Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 8

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded A20 syngeneic tumor using AIF (D39D2) XP® Rabbit mAb.
undefined Image 31: Parthanatos Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded LL/2 syngeneic tumor using AIF (D39D2) XP® Rabbit mAb.
undefined Image 32: Parthanatos Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded mouse testis using AIF (D39D2) XP® Rabbit mAb.
undefined Image 33: Parthanatos Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 8

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using AIF (D39D2) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
undefined Image 34: Parthanatos Antibody Sampler Kit
Immunoprecipitation of AIF protein from Jurkat cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is AIF (D39D2) XP® Rabbit mAb. Western blot analysis was performed using AIF (D39D2) XP® Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody.
undefined Image 35: Parthanatos Antibody Sampler Kit
Simple Western™ analysis of lysates (1 mg/mL) from Jurkat cells treated with Etoposide (25 uM, 5 hours) using PARP (46D11) Rabbit mAb #9532. The virtual lane view (left) shows the target bands (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.
undefined Image 36: Parthanatos Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 8

Cell Signaling Technology Logo
Enhanced cross-linking and immunoprecipitation (eCLIP) was performed with RNA from K-562 cells and PARP (46D11) Rabbit mAb using a protocol based on the RBP-eCLIP method from Eclipsebio. The figure shows binding across the GET4 transcript. Data is kindly provided by the laboratory of Dr. Gene Yeo and used with permission.
undefined Image 37: Parthanatos Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.