WB, IP, IHC-P, IF-IC
H
Endogenous
50
Rabbit IgG
#P26367
5080
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
| Immunohistochemistry (Paraffin) | 1:250 - 1:1000 |
| Immunofluorescence (Immunocytochemistry) | 1:100 - 1:400 |
Storage
For a carrier free (BSA and azide free) version of this product see product #58677.
Specificity / Sensitivity
Species Reactivity:
Human
Species predicted to react based on 100% sequence homology
The antigen sequence used to produce this antibody shares
100% sequence homology with the species listed here, but
reactivity has not been tested or confirmed to work by CST.
Use of this product with these species is not covered under
our
Product Performance Guarantee.
Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Pax6 protein.
Background
Paired box (PAX) proteins are a family of transcription factors that play important and diverse roles in animal development (1). Nine PAX proteins (PAX1-9) have been described in humans and other mammals. They are defined by the presence of an amino-terminal "paired" domain, consisting of two helix-turn-helix motifs, with DNA binding activity (2). PAX proteins are classified into four structurally distinct subgroups (I-IV) based on the absence or presence of a carboxy-terminal homeodomain and a central octapeptide region. Subgroup I (PAX1 and 9) contains the octapeptide but lacks the homeodomain; subgroup II (PAX2, 5, and 8) contains the octapeptide and a truncated homeodomain; subgroup III (PAX3 and 7) contains the octapeptide and a complete homeodomain; and subgroup IV (PAX4 and 6) contains a complete homeodomain but lacks the octapeptide region (2). PAX proteins play critically important roles in development by regulating transcriptional networks responsible for embryonic patterning and organogenesis (3); a subset of PAX proteins also maintain functional importance during postnatal development (4). Research studies have implicated genetic mutations that result in aberrant expression of PAX genes in a number of cancer subtypes (1-3), with members of subgroups II and III identified as potential mediators of tumor progression (2).
Pax6 has important functions in organ development. It is a key regulator of eye development (5), and mutations in Pax6 have been associated with some forms of aniridia, a congenital malformation of the eye (6). Pax6 is also involved in neuronal development, which plays an especially important role in corticogenesis (7). Within its role in the adult brain, it has recently been associated with aging, with gene occupancy studies showing increased association of Pax6 with genes associated with many aging processes in mice (8,9). Pax6 also plays another important function in development and maintenance of pancreatic β-cells (10). Driven by expression of Pdx1, both Pax6 and NGN3 expression is required for β-cell identity (11).
- Lang, D. et al. (2007) Biochem Pharmacol 73, 1-14.
- Robson, E.J. et al. (2006) Nat Rev Cancer 6, 52-62.
- Wang, Q. et al. (2008) J Cell Mol Med 12, 2281-94.
- Blake, J.A. et al. (2008) Dev Dyn 237, 2791-803.
- Shaham, O. et al. (2012) Prog Retin Eye Res 31, 351-76.
- Hingorani, M. et al. (2012) Eur J Hum Genet 20, 1011-7.
- Manuel, M.N. et al. (2015) Front Cell Neurosci 9, 70.
- Maurya, S.K. and Mishra, R. (2017) Ann Neurosci 24, 20-25.
- Maurya, S.K. and Mishra, R. (2017) J Chem Neuroanat 82, 60-64.
- Swisa, A. et al. (2017) Front Genet 8, 21.
- Mitchell, R.K. et al. (2017) J Biol Chem 292, 8892-8906.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation IHC-P: Immunohistochemistry (Paraffin) IF-IC: Immunofluorescence (Immunocytochemistry)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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