WB, IP, IF-IC, FC-FP
H M
Endogenous
228
Rabbit
#Q15154
5108
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:100 |
Immunofluorescence (Immunocytochemistry) | 1:800 |
Flow Cytometry (Fixed/Permeabilized) | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse
Species predicted to react based on 100% sequence homology
The antigen sequence used to produce this antibody shares
100% sequence homology with the species listed here, but
reactivity has not been tested or confirmed to work by CST.
Use of this product with these species is not covered under
our
Product Performance Guarantee.
Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a region near the carboxyl terminus of human PCM-1 protein. Antibodies were purified by protein A and peptide affinity chromatography.
Background
The centrosome is composed of a pair of centrioles surrounded by electron-dense pericentriolar material and functions as the microtubule-organizing center responsible for microtubule nucleation and spindle organization during cell cycle progression (1). Percentriolar material 1 (PCM-1) is a large, 228 kDa protein associated with the centrosome in a cell cycle dependent manner (2). PCM-1 localizes to small cytoplasmic granules called centrosomal satellites (3). PCM-1 is required for the assembly of several centrosomal proteins including centrin, pericentrin, ninein, NEK2, and CEP250 (4-8). Chromosomal translocations involving genes encoding PCM-1 and the tyrosine kinases Ret and Jak2 are associated with some cancers, including papillary thyroid carcinoma and myeloid leukemia (9-11).
- Zimmerman, W. and Doxsey, S.J. (2000) Traffic 1, 927-34.
- Balczon, R. et al. (1994) J Cell Biol 124, 783-93.
- Kubo, A. and Tsukita, S. (2003) J Cell Sci 116, 919-28.
- Kim, J. et al. (2008) Hum Mol Genet 17, 3796-805.
- Dammermann, A. and Merdes, A. (2002) J Cell Biol 159, 255-66.
- Li, Q. et al. (2001) J Cell Sci 114, 797-809.
- Hames, R.S. et al. (2005) Mol Biol Cell 16, 1711-24.
- Mack, G.J. et al. (1998) Arthritis Rheum 41, 551-8.
- Corvi, R. et al. (2000) Oncogene 19, 4236-42.
- Reiter, A. et al. (2005) Cancer Res 65, 2662-7.
- Bousquet, M. et al. (2005) Oncogene 24, 7248-52.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation IF-IC: Immunofluorescence (Immunocytochemistry) FC-FP: Flow Cytometry (Fixed/Permeabilized)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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