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65713
PD-L1, CD3ε, CD8α Multiplex IHC Antibody Panel
Primary Antibodies
IHC Kit

PD-L1, CD3ε, CD8α Multiplex IHC Antibody Panel #65713

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Product Description

The PD-L1, CD3ε, CD8α Multiplex IHC Antibody Panel enables researchers to simultaneously detect these targets in paraffin-embedded tissues using tyramide signal amplification. Each antibody in the panel has been validated for this approach. For recommended staining conditions optimized specifically for this antibody panel please refer to Table 1 on the Data Sheet.

Specificity / Sensitivity

Each antibody in this panel recognizes endogenous levels of its specific target protein.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PD-L1 protein, residues surrounding Glu178 of human CD3ε protein, or residues near the carboxy terminus of human CD8α protein.

Background

The field of cancer immunotherapy is focused on empowering the immune system to fight cancer. This approach has seen recent success in the clinic with targeting immune checkpoint control proteins, such as PD-1 (1,2). Despite this success, clinical biomarkers that predict response to therapeutic strategies involving PD-1 receptor blockade are still under investigation (3-5). While PD-L1 expression has been linked with an increased likelihood of response to anti-PD-1 therapy, research studies have shown that additional factors, such as tumor-immune infiltration and the ratio of effector to regulatory T cells within the tumor, could play a significant role in predicting treatment outcome (6-9).

Programmed cell death 1 ligand 1 (PD-L1) is a member of the B7 family of cell surface ligands that regulate T cell activation and immune responses. The PD-L1 ligand binds the PD-1 transmembrane receptor and inhibits T cell activation. PD-L1 is expressed in several tumor types, including melanoma, ovary, colon, lung, breast, and renal cell carcinomas (10-12).

CD3 (Cluster of Differentiation 3) is a multiunit protein complex that directly associates with the T cell receptor (TCR). CD3 is composed of four polypeptides (ζ, γ, ε and δ), each of which contains at least one immunoreceptor tyrosine-based activation motif (ITAM) (13). Engagement of TCR complex with foreign antigens induces tyrosine phosphorylation in the ITAM motifs and phosphorylated ITAMs function as docking sites for signaling molecules such as ZAP-70 and p85 subunit of PI-3 kinase (14,15).

CD8 (Cluster of Differentiation 8) is a disulphide-linked heterodimer consisting of α and β subunits. On T cells, CD8 is the coreceptor for the TCR, and these two distinct structures recognize the Antigen–Major Histocompatibility Complex (MHC). CD8 ensures specificity of the TCR–antigen interaction, prolongs the contact between the T cell and the antigen presenting cell, and the α chain recruits the tyrosine kinase Lck, which is essential for T cell activation (16).

  1. Topalian, S.L. et al. (2012) N Engl J Med 366, 2443-54.
  2. Piccinini, M. et al. (2014) Comput Methods Biomech Biomed Engin 17, 1403-17.
  3. Chakravarti, N. and Prieto, V.G. (2015) Transl Lung Cancer Res 4, 743-51.
  4. Sholl, L.M. et al. (2016) Arch Pathol Lab Med , .
  5. Carbognin, L. et al. (2015) PLoS One 10, e0130142.
  6. Tokito, T. et al. (2016) Eur J Cancer 55, 7-14.
  7. Tumeh, P.C. et al. (2014) Nature 515, 568-71.
  8. Feng, Z. et al. (2015) J Immunother Cancer 3, 47.
  9. Baine, M.K. et al. (2015) Oncotarget 6, 24990-5002.
  10. Dong, H. et al. (2002) Nat Med 8, 793-800.
  11. Thompson, R.H. et al. (2006) Cancer Res 66, 3381-5.
  12. Pardoll, D.M. (2012) Nat Rev Cancer 12, 252-64.
  13. Pitcher, L.A. and van Oers, N.S. (2003) Trends Immunol. 24, 554-560.
  14. Osman, N. et al. (1996) Eur. J. Immunol. 26, 1063-1068.
  15. Hatada, M.H. et al. (1995) Nature 377, 32-38.
  16. Zamoyska, R. (1994) Immunity 1, 243-46.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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