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2226
Phospho-Caspase-9 (Thr125) Antibody
Primary Antibodies

Phospho-Caspase-9 (Thr125) Antibody #2226

This product is discontinued

Storage:

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Phospho-Caspase-9 (Thr125) Antibody detects endogenous levels of caspase-9 only when phosphorylated at threonine 125.

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr125 of human caspase-9

Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family (1,2). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf-1. Apaf-1 mediated activation of caspase-9 involves intrinsic proteolytic processing resulting in cleavage at Asp315 and producing a p35 subunit. Another cleavage occurs at Asp330 producing a p37 subunit that can serve to amplify the apoptotic response (3-6). Cleaved caspase-9 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, which leads to apoptosis (7-10).

Caspase-9 is phosphorylated at Thr125 through the p44/42 MAPK pathway, and this phosphorylation is associated with inhibition of caspase activation (6).

  1. Duan, H. et al. (1996) J. Biol. Chem. 271, 16720-16724.
  2. Srinivasula, S. M. et al. (1996) J. Biol. Chem. 271, 27099-27106.
  3. Liu, X. et al. (1996) Cell 86, 147-157.
  4. Li, P. et al. (1997) Cell 91, 479-489.
  5. Zou, H. et al. (1999) J. Biol. Chem. 274, 11549-11556.
  6. Srinivasula, S.M. et al. (1998) Mol Cell 1, 949-57.
  7. Deveraux, Q. L. et al. (1998) EMBO J. 17, 2215-2223.
  8. Slee, E. A. et al. (1999) J. Cell Biol. 144, 281-292.
  9. Sun, X.M. et al. (1999) J Biol Chem 274, 5053-60.
  10. MacFarlane, M. et al. (1997) J. Cell Biol. 137, 469-479.
  11. Ajimura, M. et al. (1993) Genetics 133, 51-66.
Entrez-Gene Id
842
Swiss-Prot Acc.
P55211
For Research Use Only. Not For Use In Diagnostic Procedures.

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