WB
All
Endogenous
Rabbit
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
All Species Expected
Source / Purification
MultiMab® rabbit monoclonal mix antibodies are prepared by combining individual rabbit monoclonal clones in optimized ratios for the approved applications. Each antibody in the mix is carefully selected based on motif recognition and performance in multiple assays. Each mix is engineered to yield the broadest possible coverage of the modification being studied while ensuring a high degree of specificity for the modification or motif.
Background
Casein Kinase II (CK2) is a highly conserved, ubiquitously expressed, and constitutively active tetrameric Ser/Thr protein kinase with hundreds of substrates participating in the regulation of a variety of cellular processes including cell cycle progression, apoptosis, transcription, inflammation, and the DNA damage response. Research studies have implicated CK2 in roles related to viral infection, cancer, and other diseases (1-5). CK2 substrates contain multiple acidic residues (Asp and Glu) located downstream of the phosphorylated Ser or Thr residue. The consensus sequence for CK2 substrates is pS/pTD/EXD/E with the most crucial residue at the +3 position followed by the residue at the +1 position (6).
- Pinna, L.A. and Allende, J.E. (2009) Cell Mol Life Sci 66, 1795-9.
- St-Denis, N.A. and Litchfield, D.W. (2009) Cell Mol Life Sci 66, 1817-29.
- Trembley, J.H. et al. (2009) Cell Mol Life Sci 66, 1858-67.
- Perez, D.I. et al. (2011) Med Res Rev 31, 924-54.
- Dominguez, I. et al. (2009) Cell Mol Life Sci 66, 1850-7.
- Meggio, F. and Pinna, L.A. (2003) FASEB J 17, 349-68.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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