WB, IP
H R Mk
Endogenous
105
Rabbit
#O00418
29904
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser366 of human eEF2k. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Eukaryotic elongation factor 2 kinase (eEF2k) phosphorylates and inactivates eEF2, resulting in the inhibition of peptide-chain elongation (1). eEF2k is normally dependent on Ca2+ ions and calmodulin (2,3). It can be activated by PKA in response to elevated cAMP levels (4-6), which are generally increased in stress- or starvation-related conditions. eEF2k can also be regulated in response to a wide range of stimuli that promote cell growth and protein synthesis. This involves the phosphorylation of eEF2k by p90RSK and p70 S6 kinase at Ser366 or by SAPK4/p38delta at Ser359, leading to the inactivation of eEF2k (7,8), which facilitates the dephosphorylation of eEF2, and thus promotes translation.
- Ryazanov, A.G. et al. (1997) Proc. Natl. Acad. Sci. USA 94, 4884-4889.
- Nairn, A.C. et al. (1985) Proc. Natl. Acad. Sci. USA 82, 7839-7943.
- Palfrey, H.C. et al. (1987) J. Biol. Chem. 262, 9785-9792.
- Redpath, N.T. et al. (1993) Biochem. J. 293, 31-34.
- Diggle, T.A. et al. (1998) Biochem. J. 336, 525-529.
- Hovland, R. et al. (1999) FEBS Lett. 444, 97-101.
- Wang, X. et al. (2001) EMBO J. 20, 4370-4379.
- Knebel, A. et al. (2001) EMBO J. 20, 4360-4369.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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