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PhosphoSitePlus® Resource

  • Additional protein information
  • Analytical tools

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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-EGF Receptor (Tyr1068) (D7A5) XP® Rabbit mAb 3777 40 µl
Western Blotting Immunohistochemistry Immunofluorescence Flow Cytometry
H M R Mk 175 Rabbit IgG
Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb 4060 40 µl
Western Blotting Immunoprecipitation Immunohistochemistry Immunofluorescence Flow Cytometry
H M R Hm Mk Dm Z B 60 Rabbit IgG
Phospho-Gab1 (Tyr627) (C32H2) Rabbit mAb 3233 40 µl
Western Blotting
H 110 Rabbit IgG
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb 4370 40 µl
Western Blotting Immunoprecipitation Immunohistochemistry Immunofluorescence Flow Cytometry
H M R Hm Mk Mi Dm Z B Dg Pg Sc 44, 42 Rabbit IgG
Phospho-Shc (Tyr239/240) Antibody 2434 40 µl
Western Blotting
H M R 50, 55, 70 Rabbit 
Phospho-Stat5 (Tyr694) (D47E7) XP® Rabbit mAb 4322 40 µl
Western Blotting Immunofluorescence Flow Cytometry
H M 90 Rabbit IgG
Phospho-c-Cbl (Tyr700) (D16D7) Rabbit mAb 8869 40 µl
Western Blotting Immunoprecipitation
H 120 Rabbit IgG
Phospho-PLCγ1 (Tyr783) (D6M9S) Rabbit mAb 14008 40 µl
Western Blotting Immunoprecipitation Immunofluorescence Flow Cytometry
H M 155 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting
Goat 

Product Description

The Phospho-EGF Receptor Pathway Sampler Kit provides an economical means to evaluate the activation status of multiple members of the EGF receptor pathway, including phosphorylated EGF receptor, Stat5, c-Cbl, Shc, Gab1, PLCγ1, Akt and p44/42 MAPK. The kit includes enough primary and secondary antibodies to perform four western blot experiments.


Specificity / Sensitivity

Each antibody in the Phospho-EGF Receptor Pathway Sampler Kit recognizes the phosphorylated form of its specific target. Phospho-EGF Receptor (Tyr1068) (D7A5) XP® Rabbit mAb may cross-react weakly with other tyrosine-phosphorylated proteins. Phospho-Gab1 (Tyr627) (C32H2) Rabbit mAb may cross-react with phosphorylated Gab2, Gab3, or activated receptor tyrosine kinases. Phospho-Shc (Tyr239/240) Antibody may cross-react with activated EGF receptor protein.


Source / Purification

Activation state polyclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Tyr239/240 of human Shc. Polyclonal antibodies are purified by protein A and peptide affinity chromatography. Rabbit monoclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Tyr700 of c-Cbl, Tyr1068 of human EGF receptor, Tyr694 of Stat5a, Tyr627 of human Gab1, Ser473 of human Akt, Thr202/Tyr204 of human p44 MAP kinase, or Tyr783 of human PLCγ1 protein.

The epidermal growth factor (EGF) receptor is a transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Ligand binding results in receptor dimerization, autophosphorylation, activation of downstream signaling, internalization, and lysosomal degradation (1,2). Phosphorylation of EGF receptor (EGFR) at Tyr845 in the kinase domain is implicated in stabilizing the activation loop, maintaining the active state enzyme, and providing a binding surface for substrate proteins (3,4). c-Src is involved in phosphorylation of EGFR at Tyr845 (5). The SH2 domain of PLCγ binds at phospho-Tyr992, resulting in activation of PLCγ-mediated downstream signaling (6). Phosphorylation of EGFR at Tyr1045 creates a major docking site for the adaptor protein c-Cbl, leading to receptor ubiquitination and degradation following EGFR activation (7,8). The GRB2 adaptor protein binds activated EGFR at phospho-Tyr1068 (9). A pair of phosphorylated EGFR residues (Tyr1148 and Tyr1173) provide a docking site for the Shc scaffold protein, with both sites involved in MAP kinase signaling activation (2). Phosphorylation of EGFR at specific serine and threonine residues attenuates EGFR kinase activity. EGFR carboxy-terminal residues Ser1046 and Ser1047 are phosphorylated by CaM kinase II; mutation of either of these serines results in upregulated EGFR tyrosine autophosphorylation (10).


1.  Hackel, P.O. et al. (1999) Curr Opin Cell Biol 11, 184-9.

2.  Zwick, E. et al. (1999) Trends Pharmacol Sci 20, 408-12.

3.  Cooper, J.A. and Howell, B. (1993) Cell 73, 1051-4.

4.  Hubbard, S.R. et al. (1994) Nature 372, 746-54.

5.  Biscardi, J.S. et al. (1999) J Biol Chem 274, 8335-43.

6.  Emlet, D.R. et al. (1997) J Biol Chem 272, 4079-86.

7.  Levkowitz, G. et al. (1999) Mol Cell 4, 1029-40.

8.  Ettenberg, S.A. et al. (1999) Oncogene 18, 1855-66.

9.  Rojas, M. et al. (1996) J Biol Chem 271, 27456-61.

10.  Feinmesser, R.L. et al. (1999) J Biol Chem 274, 16168-73.


Entrez-Gene Id 207 , 208 , 10000 , 867 , 1956 , 5595 , 5594 , 2549 , 5335 , 6464 , 6776 , 6777
Swiss-Prot Acc. P31749 , P31751 , Q9Y243 , P22681 , P00533 , P27361 , P28482 , Q13480 , P19174 , P29353 , P42229 , P51692


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 5,675,063.

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Phospho-EGF Receptor Pathway Antibody Sampler Kit