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12596
Phospho-FLT3 Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Phospho-FLT3 Antibody Sampler Kit #12596

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To Purchase # 12596

Product Description

The Phospho-FLT3 Antibody Sampler Kit provides an economical means of evaluating the FLT3 tyrosine kinase and several phosphorylation sites that are involved in its activation. The kit includes enough antibody to perform four western blot experiments with each primary antibody.

Specificity / Sensitivity

The phospho-FLT3 antibodies recognize the phosphorylated form of FLT3 at the indicated sites. The Phospho-FLT3 (Tyr591) (33G6) Rabbit mAb and Phospho-FLT3 (Tyr969) (C24D9) Rabbit mAb may cross-react with other tyrosine-phosphorylated proteins. The Phospho-FLT3 (Tyr842) (10A8) Rabbit mAb may cross-react with other tyrosine phosphorylated family members. The control FLT3 antibody recognizes both the phosphorylated and nonphosphorylated forms of this kinase.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Tyr589/591, Tyr591, Tyr842 or Tyr969 of human FLT3. The FLT3 antibody is produced by immunizing animals with a synthetic peptide surrounding Ser740 of human FLT3.

Background

FMS-related tyrosine kinase 3 (FLT3, also called Flk2), is a member of the type III receptor tyrosine kinase family, which includes c-Kit, PDGFR and M-CSF receptors. FLT3 is expressed on early hematopoietic progenitor cells and supports growth and differentiation within the hematopoietic system (1,2). FLT3 is activated after binding with its ligand FL, which results in a cascade of tyrosine autophosphorylation and tyrosine phosphorylation of downstream targets (3). The p85 subunit of PI3 kinase, SHP2, GRB2 and Shc are associated with FLT3 after FL stimulation (4-6). Tyr589/591 is located in the juxtamembrane region of FLT3 and may play an important role in regulation of FLT3 tyrosine kinase activity. Somatic mutations of FLT3 consisting of internal tandem duplications (ITDs) occur in 20% of patients with acute myeloid leukemia (7).

  1. Shurin, M.R. et al. (1998) Cytokine Growth Factor Rev 9, 37-48.
  2. Naoe, T. et al. (2001) Cancer Chemother Pharmacol 48 Suppl 1, S27-30.
  3. Namikawa, R. et al. (1996) Stem Cells 14, 388-95.
  4. Beslu, N. et al. (1996) J Biol Chem 271, 20075-81.
  5. Zhang, S. and Broxmeyer, H.E. (2000) Biochem Biophys Res Commun 277, 195-9.
  6. Zhang, S. et al. (1999) J Leukoc Biol 65, 372-80.
  7. Mizuki, M. et al. (2000) Blood 96, 3907-14.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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