Phospho-GLI1 (Thr1074) (F9W5G) Rabbit Monoclonal Antibody #27906
- WB
- IP
Product Specifications
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 160 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
GLI1 phosphorylation by upstream kinases can control its stability, localization, and transcriptional activity. AMPK phosphorylates GLI1 at three sites, Ser102, Ser408, and Thr1074, increasing GLI1 cytoplasmic localization and targeting it for degradation via the β-TrCP/SCF E3 ligase pathway (6,7). IKKβ promotes GLI1 stability by phosphorylating it at a cluster of serine residues (Ser543-Ser548) and Thr1071, inhibiting ITCH-mediated ubiquitination (8). MEKK3 phosphorylates GLI1 n-terminus (Ser201, Ser204, Ser243) and c-terminus (Ser968, Thr1074, Ser1078), preventing GLI1 nuclear accumulation, increasing GLI1 association with Hedgehog-regulator SUFU, and inhibiting GLI1 association with target promoters (9).
- Kinzler, K.W. et al. (1987) Science 236, 70-3.
- Ruppert, J.M. et al. (1991) Mol Cell Biol 11, 1724-8.
- Kinzler, K.W. et al. (1988) Nature 332, 371-4.
- Ingham, P.W. and McMahon, A.P. (2001) Genes Dev 15, 3059-87.
- McMahon, A.P. et al. (2003) Curr Top Dev Biol 53, 1-114.
- Li, Y.H. et al. (2015) Cell Rep 12, 599-609.
- Zhang, R. et al. (2017) Oncotarget 8, 49869-49881.
- Agarwal, N.K. et al. (2016) Blood 127, 605-15.
- Lu, J. et al. (2018) Oncogene 37, 3864-3878.
Alternate Names
GLI; GLI family zinc finger 1; GLI-Kruppel family member GLI1; GLI1; Glioma-associated oncogene; glioma-associated oncogene 1; glioma-associated oncogene family zinc finger 1; glioma-associated oncogene homolog 1 (zinc finger protein); Oncogene GLI; PAPA8; PPD1; Zinc finger protein GLI1
Limited Uses
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