Revision 1
#88583
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W
Reactivity:
H
Sensitivity:
Endogenous
MW (kDa):
185
Source/Isotype:
Rabbit
UniProt ID:
#P04626
Entrez-Gene Id:
2064
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity/Sensitivity
Species predicted to react based on 100% sequence homology
Source / Purification
Background
Thr686 is located in the cytoplasmic juxtamembrane region of ErbB2. PKC, PKA, and PKG II kinases have been reported to phosphorylate ErbB2 at this site, but the functional consequences of phosphorylation may be context dependent. For example, one research study reported that phosphorylation of ErbB2 at Thr686 enhanced ligand-induced receptor activation (6), while a separate research study reported inhibitory effects of phosphorylation on ErbB2 activation (8).
Background References
- Muthuswamy, S.K. et al. (1999) Mol Cell Biol 19, 6845-57.
- Qian, X. et al. (1994) Proc Natl Acad Sci USA 91, 1500-4.
- Dittadi, R. and Gion, M. (2000) J Natl Cancer Inst 92, 1443-4.
- Klapper, L.N. et al. (2000) Cancer Res 60, 3384-8.
- Kwon, Y.K. et al. (1997) J Neurosci 17, 8293-9.
- Monje, P.V. et al. (2008) J Biol Chem 283, 34087-100.
- Ouyang, X. et al. (1998) Carcinogenesis 19, 2013-9.
- Zhu, M. et al. (2016) Mol Med Rep 13, 1909-13.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting
Cross-Reactivity Key
H: Human
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Revision 1