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9938
Phospho-MAPK Family Rabbit mAb Sampler Kit
Primary Antibodies

Phospho-MAPK Family Rabbit mAb Sampler Kit #9938

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# Product Name
To Purchase # 9938

Kit Usage Information

Protocols
  • 9903:

Product Description

Phospho-MAPK Family Rabbit mAb Sampler Kit contains 40 µl of each primary rabbit monoclonal antibody [Phospho-p44/42 MAPK (Thr202/Tyr204), Phospho-SAPK/JNK (Thr183/Tyr185), Phospho-p38 MAPK (Thr180/Tyr182)], 50 µl of anti-rabbit IgG secondary antibody (HRP conjugated) and 0.3 mg of the MEK1/2 Inhibitor (U0126).

Specificity / Sensitivity

Phospho-p44/42 MAPK, Phospho-SAPK/ JNK and Phospho-p38 MAPK Rabbit mAbs recognize only the phosphorylated form of p44/42 MAPK, SAPK/JNK and p38 MAPK, respectively. They do not cross-react with other MAPK family members.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptides corresponding to residues surrounding Thr202/Tyr204 of human p44 MAPK (Erk1), Thr183/Tyr185 of human SAPK or Thr180/Tyr182 of human p38. For MEK1/2 Inhibitor (U0126) #9903 information, see page 3.

Background

p44/42 MAPK (Erk1/2), SAPK/JNK, and p38 MAPK function in protein kinase cascades that play a critical role in the regulation of cell growth, differentiation, and control of cellular responses to cytokines and stress. p44/42 MAPK is activated by growth and neurotrophic factors. Activation occurs through phosphorylation of threonine and tyrosine residues (Thr202 and Tyr204 in human Erk1) at the sequence T*EY* by a single upstream MAP kinase kinase (MEK). SAPK/JNK and p38 MAPK are activated by inflammatory cytokines and by a wide variety of cellular stresses. Activation of SAPK/JNK occurs via phosphorylation at Thr183 and Tyr185 by the dual specificity enzyme SEK/MKK4. Both MKK3 and SEK phosphorylate p38 MAPK on tyrosine and threonine at the sequence T*GY* to activate p38 MAP kinase (1-5).

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  2. Garrington, T.P. and Johnson, G.L. (1999) Curr. Opin. Cell. Biol. 11, 211-218.
  3. Schaeffer, H.J. and Weber, M.J. (1999) Mol. Cell. Biol. 19, 2435-2444.
  4. Whitmarsh, A.J. and Davis, R.J. (1998) Trends Biochem Sci 23, 481-5.
  5. Cobb, M.H. (1999) Prog. Biophys. Mol. Biol. 71, 479-500.

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For Research Use Only. Not For Use In Diagnostic Procedures.

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