WB
H
Endogenous
70
Rabbit
#Q99640
9088
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser83 of human Myt1. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Entry of all eukaryotic cells into mitosis is regulated by activation of cdc2 kinase. The critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Thr14 (1,2). Phosphorylation at Tyr15 and Thr14 and inhibition of cdc2 is carried out by Wee1 and Myt1 protein kinases, while Tyr15 dephosphorylation and activation of cdc2 is carried out by the cdc25 phosphatase (1,3,4). Hyperphosphorylation and inactivation of Myt1 in mitosis suggests that one or more kinases activated at the G2/M transition negatively regulates Myt1 activity. Kinases shown to phosphorylate Myt1 include cdc2, p90RSK, Akt, and Plk1 (5-7).
Although Akt has been shown to phosphorylate Asterina (starfish) Myt1 at a consensus Akt phosphorylation site (7), the orthologous site, Ser83, in human Myt1 may be phosphorylated by a different kinase.
- Watanabe, N. et al. (1995) EMBO J. 14, 1878-1891.
- Hunter, T. (1995) Cell 80, 225-236.
- Galaktionov, K. et al. (1995) Genes Dev 9, 1046-58.
- McGowan, C.H. and Russell, P. (1993) EMBO J 12, 75-85.
- Booher, R.N. et al. (1997) J Biol Chem 272, 22300-6.
- Palmer, A. et al. (1998) EMBO J 17, 5037-47.
- Nakajima, H. et al. (2003) J Biol Chem 278, 25277-80.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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