|MW (kDa)||42, 44|
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Phospho-p44/42 (Thr202/Tyr204) (E10) Monoclonal Antibody can be used in high throughput kinase assays and drug discovery applications. It detects peptides derived from p44/42 MAPK phosphorylated at threonine 202 and tyrosine 204.
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide correspondig to residues surrounding Thr202/Tyr204 of human p44 MAP kinase. Antibody is purified by protein G affinity chromatography.
Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs, such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines (1-3), and research investigators consider it an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family, as well as Mos and Tpl2/COT. MEK1 and MEK2 are the primary MAPKKs in this pathway (5,6). MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK (7) and the transcription factor Elk-1 (8,9). p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs (10), along with MEK inhibitors, such as U0126 and PD98059.
Description: This antibody is formulated in PBS (no BSA/no glycerol) and quality controlled for ELISA and other drug discovery applications. This is a sample antibody and intended for use by drug discovery scientists.
Please direct inquiries for bulk pricing of this antibody, peptide substrates, or protocols to firstname.lastname@example.org.
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