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4081
Phospho-PFK-2 (Ser466) Antibody
Primary Antibodies
Polyclonal Antibody

Phospho-PFK-2 (Ser466) Antibody #4081

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Supporting Data

REACTIVITY
SENSITIVITY
MW (kDa) 64
SOURCE Rabbit

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • C&R-CUT&RUN
  • C&T-CUT&Tag
  • DB-Dot Blot
  • eCLIP-eCLIP
  • IF-Immunofluorescence
  • F-Flow Cytometry

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • GP-Guinea Pig
  • Rab-Rabbit
  • All-All Species Expected

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Phospho-PFK-2 (Ser466) Antibody detects endogenous Ser466 phosphorylated cardiac PFK-2, as well as the kidney isoform. This antibody may also reacts with the inducible PFK-2, as well as the brain, placental isoforms.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding serine 466 of human PFK-2 (cardiac isoform). Antibodies are purified by protein A and peptide affinity chromatography.

Background

PFK-2 (6-phosphofructo-2-kinase/fructose-2,6-bisphosphate 2-phosphatase) is a bifunctional enzyme catalyzing the synthesis and the degradation of fructose 2,6-bisphosphate, which is the potent stimulator of 6-phosphofructo-1-kinase and glycolysis. Several tissue specific isoforms have been reported, which have conserved catalytic core flanked by variable N- and C-terminal regulatory domains (1). It has been reported that Ser466 of the cardiac PFK-2, and Ser461 of the placental PFK-2 (also known as inducible PFK-2, iPFK-2) are phosphorylated by AMPK as well as by other kinases, which stimulates the activity of PFK-2 and glycolysis during ischemia and hypoxia (2-4). iPFK-2 process a typical oncogene-like regulatory element in its mRNA, which contributes to the high level expression of iPFK-2 and enhanced glycolysis in cancer cells (5).

Pathways

Explore pathways related to this product.

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.
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