Revision 1
#5445
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W, IP, FC-FP
Reactivity:
H
Sensitivity:
Endogenous
MW (kDa):
160
Source/Isotype:
Rabbit IgG
UniProt ID:
#O15357
Entrez-Gene Id:
3636
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
| Flow Cytometry (Fixed/Permeabilized) | 1:1600 |
Storage
Specificity/Sensitivity
Source / Purification
Background
SHIP2, a homolog of SHIP1, is highly expressed in heart, skeletal muscle and placenta (7). SHIP2 negatively regulates insulin signaling (8) and polymorphisms in SHIP2 have been linked to hyperglycemia (9). Recent studies also suggest SHIP2 as a therapeutic target for the treatment of both obesity and type 2 diabetes (10,11). Tyr1135 is phosphorylated in human cancer cells (12-15).
Background References
- Tridandapani, S. et al. (1997) Mol Cell Biol 17, 4305-11.
- Liu, L. et al. (1997) J Biol Chem 272, 8983-8.
- Malbec, O. et al. (2001) J Biol Chem 276, 30381-91.
- Carver, D.J. et al. (2000) Blood 96, 1449-56.
- Scharenberg, A.M. et al. (1998) EMBO J 17, 1961-72.
- Sattler, M. et al. (2001) J Biol Chem 276, 2451-8.
- Pesesse, X. et al. (1997) Biochem Biophys Res Commun 239, 697-700.
- Wada, T. et al. (2001) Mol Cell Biol 21, 1633-46.
- Ishida, S. et al. (2006) Pancreas 33, 63-7.
- Liang, X. et al. (2006) Proteomics 6, 4554-64.
- Dyson, J.M. et al. (2005) Int J Biochem Cell Biol 37, 2260-5.
- Goss, V.L. et al. (2006) Blood 107, 4888-97.
- Guo, A. et al. (2008) Proc Natl Acad Sci U S A 105, 692-7.
- Rikova, K. et al. (2007) Cell 131, 1190-203.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting IP: Immunoprecipitation FC-FP: Flow Cytometry (Fixed/Permeabilized)
Cross-Reactivity Key
H: Human
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Revision 1