# | Product Name | |||
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12656 | SMAD 1/5/9 Antibody Sampler Kit |
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12747 | SMAD2/3 Antibody Sampler Kit |
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Product Information
Phospho-specific monoclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Ser465/467 of human Smad2, Ser423/425 of Smad3, and Ser463/465 of human Smad5. Total Smad1, Smad2 and Smad3 monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Ser190 of human Smad1 and near the amino termini of mouse Smad2 and human Smad3. Polyclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to the residues surrounding surrounding Pro278 of human Smad4, Gln252 of human Smad5, and Cys55 of human Smad6. Antibodies are purified by protein A and peptide affinity chromatography.
Members of the SMAD family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of SMADs have been defined: the receptor-regulated SMADs (R-SMADs), which include SMAD1, 2, 3, 5, and 9; the common-mediator SMAD (co-SMAD), SMAD4; and the antagonistic or inhibitory SMADs (I-SMADs), SMAD6 and 7 (1-5). Activated type I receptors associate with specific R-SMADs and phosphorylate them on a conserved carboxy-terminal SSXS motif. The phosphorylated R-SMADs dissociate from the receptor and form a heteromeric complex with SMAD4, initiating translocation of the heteromeric SMAD complex to the nucleus. Once in the nucleus, SMADs recruit a variety of DNA binding proteins that function to regulate transcriptional activity (6-8).
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