Revision 2

#89892

Store at -20C

Phospho-Tau (Ser214/T217) Signaling Antibody Sampler Kit

1 Kit

(8 x 20 microliters)

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Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

Web:

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Product Includes Product # Quantity Mol. Wt Isotype/Source
Tau (D1M9X) Rabbit Monoclonal Antibody4668720 µl50-80 kDaRabbit IgG
Phospho-Tau (Ser214) (D1Q2X) Rabbit Monoclonal Antibody7734820 µl50-80 kDaRabbit IgG
Phospho-Tau (Thr217) (E9Y4S) Rabbit Monoclonal Antibody5162520 µl50-80 kDaRabbit IgG
Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) Rabbit Monoclonal Antibody451120 µl43 kDaRabbit IgG
Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit Monoclonal Antibody466820 µl46, 54 kDaRabbit IgG
Phospho-p70 S6 Kinase (Thr389) (D5U1O) Rabbit Monoclonal Antibody9759620 µl70, 85 kDaRabbit IgG
p38 MAPK (D13E1) Rabbit Monoclonal Antibody869020 µl40 kDaRabbit IgG
p70 S6 Kinase (E8K6T) Rabbit Monoclonal Antibody3447520 µl70, 85 kDaRabbit IgG
Anti-rabbit IgG, HRP-linked Antibody7074100 µlGoat

Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.

Description

The Phospho-Tau (Ser214/T217) Signaling Antibody Sampler Kit provides an economical means of detecting the signaling cascade leading to tau phosphorylation at Ser214 and Thr217, using phospho-specific and control antibodies. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Background

Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Six isoforms with different amino-terminal inserts and different numbers of tandem repeats near the carboxy terminus have been identified, and tau is hyperphosphorylated at approximately 25 sites by Erk, glycogen synthase kinase-3 (GSK-3), and CDK5 (1,2). Phosphorylation decreases the ability of tau to bind to microtubules. Neurofibrillary tangles are a major hallmark of Alzheimer's disease (AD); these tangles are bundles of paired helical filaments (PHFs) composed of hyperphosphorylated tau. Furthermore, research studies have shown that inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1,3).

Numerous kinases, including p38 MAPK, JNK, CDK5, GSK3β, and SGK1, have been shown to phosphorylate tau at Ser214, which is found in AD and dementia with Lewy bodies (4-9). p38 MAPK participates in a signaling cascade controlling cellular responses to cytokines and stress and is activated by phosphorylation at Thr180 and Tyr182, allowing for downstream tau Ser214 phosphorylation (10-13). Another kinase that phosphorylates the Ser214 site on tau is the stress-activated protein kinase/Jun-amino-terminal kinase (SAPK/JNK). SAPK/JNK is preferentially activated by a variety of environmental stresses, including UV and gamma radiation, ceramides, inflammatory cytokines, and, in some instances, growth factors and GPCR agonists, which lead to phosphorylation of Thr183 and Tyr185 (14-19).

Tau phosphorylation at Thr217 has been identified as a highly specific biomarker of AD. Phosphorylated tau at Thr217 can be detected in cerebrospinal fluid in both preclinical and advanced stages of AD and has been shown to successfully differentiate between AD and non-AD neurodegenerative diseases (20,21). One kinase identified to phosphorylate the Thr217 site on tau is p70 S6 kinase (S6K1). S6K1 is a mitogen activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression (22,23). Phosphorylation of Thr389 most closely correlates with S6K1 activity in vivo (24).

Background References

  1. Johnson, G.V. and Stoothoff, W.H. (2004) J Cell Sci 117, 5721-9.
  2. Hanger, D.P. et al. (1998) J Neurochem 71, 2465-76.
  3. Bramblett, G.T. et al. (1993) Neuron 10, 1089-99.
  4. Illenberger, S. et al. (1998) Mol Biol Cell 9, 1495-512.
  5. Götz, J. et al. (2001) Science 293, 1491-5.
  6. Yang, Y.C. et al. (2006) Mol Cell Biol 26, 8357-70.
  7. Liu, F. et al. (2006) FEBS Lett 580, 6269-74.
  8. Zhu, B. et al. (2010) Am J Physiol Lung Cell Mol Physiol 299, L493-501.
  9. Duka, V. et al. (2013) PLoS One 8, e75025.
  10. Rouse, J. et al. (1994) Cell 78, 1027-37.
  11. Han, J. et al. (1994) Science 265, 808-11.
  12. Lee, J.C. et al. Nature 372, 739-46.
  13. Freshney, N.W. et al. (1994) Cell 78, 1039-49.
  14. Davis, R.J. (1999) Biochem Soc Symp 64, 1-12.
  15. Ichijo, H. (1999) Oncogene 18, 6087-93.
  16. Kyriakis, J.M. and Avruch, J. (2001) Physiol Rev 81, 807-69.
  17. Kyriakis, J.M. (1999) J Biol Chem 274, 5259-62.
  18. Leppä, S. and Bohmann, D. (1999) Oncogene 18, 6158-62.
  19. Whitmarsh, A.J. and Davis, R.J. (1998) Trends Biochem Sci 23, 481-5.
  20. Barthélemy, N.R. et al. (2020) Alzheimers Res Ther 12, 26.
  21. Janelidze, S. et al. (2020) Nat Commun 11, 1683.
  22. Pullen, N. and Thomas, G. (1997) FEBS Lett 410, 78-82.
  23. Dufner, A. and Thomas, G. (1999) Exp Cell Res 253, 100-9.
  24. Weng, Q.P. et al. (1998) J Biol Chem 273, 16621-9.

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Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.