WB, IP, IF-IC
H M
Endogenous
35
Rabbit IgG
#Q99453
8929
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunofluorescence (Immunocytochemistry) | 1:200 - 1:800 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PHOX2B protein.
Background
PHOX2B (also called neuroblastoma PHOX, NBPhox) is a homeodomain transcription factor expressed primarily in neuronal cells of the peripheral autonomic nervous system (1). Research studies in animal models have shown that PHOX2B expression is specifically required for the generation and maintenance of neuroblasts derived from the neural crest (2). PHOX2B expression has likewise been identified as a sensitive and specific marker of undifferentiated neuroblastoma tumors, including metastatic neuroblastoma cells in bone marrow (3-5). Specific mutations in the gene encoding PHOX2B have been directly linked with familial neuroblastoma cases (6) in addition to autonomic nerve disorders that can co-occur with neuroblastoma (e.g., Hirschsprung's disease, congenital central hypoventilation syndrome) (7). These findings underscore the central importance of PHOX2B to development and function of the peripheral autonomic nervous system.
- Brunet, J.F. and Pattyn, A. (2002) Curr Opin Genet Dev 12, 435-40.
- Stanke, M. et al. (1999) Development 126, 4087-94.
- Hata, J.L. et al. (2015) Arch Pathol Lab Med 139, 543-6.
- Hung, Y.P. et al. (2017) Histopathology 71, 786-794.
- Warren, M. and Shimada, H. (2016) Pediatr Dev Pathol 19, 254-5.
- Bourdeaut, F. et al. (2005) Cancer Lett 228, 51-8.
- Gaultier, C. et al. (2005) Pediatr Res 58, 1-6.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation IF-IC: Immunofluorescence (Immunocytochemistry)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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