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9655
PI3 Kinase Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

PI3 Kinase Antibody Sampler Kit #9655

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PI3 Kinase Antibody Sampler Kit: Image 1

Western blot analysis of extracts from NIH/3T3-Src cells, untreated or treated with lambda phosphatase and from C2C12 cells, untreated or treated with H2O2, using Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) Antibody.

PI3 Kinase Antibody Sampler Kit: Image 2

Western blot analysis of extracts from Jurkat and NIH/3T3 cells, using PI3 Kinase p85 (19H8) Rabbit mAb.

PI3 Kinase Antibody Sampler Kit: Image 3

Western blot analysis of extracts from HeLa cells and neonatal mouse brain using PI3 Kinase p110α (C73F8) Rabbit mAb.

PI3 Kinase Antibody Sampler Kit: Image 4

Western blot analysis of extracts from MCF-7 and K-562 cells using PI3 Kinase p110β (C33D4) Rabbit mAb.

PI3 Kinase Antibody Sampler Kit: Image 5

Western blot analysis of extracts from 293 and RN33B cells using PI3 Kinase Class III (D4E2) Rabbit mAb.

PI3 Kinase Antibody Sampler Kit: Image 6

Western blot analysis of extracts from NIH/3T3, Jurkat and K562 cells using PI3 Kinase p110γ (D55D5) Rabbit mAb.

PI3 Kinase Antibody Sampler Kit: Image 7

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

To Purchase # 9655T
Product # Size Price
9655T
1 Kit  (6 x 20 µl) $ 445

Product Description

The PI3 Kinase Sampler Kit provides an economical means of studying PI3 kinase subunits in cells. The kit contains enough primary and secondary antibodies to perform two Western blot experiments per primary antibody.

Specificity / Sensitivity

Each antibody in the PI3 Kinase Antibody Sampler Kit recognizes only its specific target and does not cross-react with other family members. Each total antibody detects endogenous levels of its target protein. Phospho-PI3K p85 (Tyr458)/p55 (Tyr199) Antibody detects endogenous levels of p85/p55 only when phosphorylated at Tyr458/Tyr199.

Source / Purification

Phospho-specific polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr458 of mouse p85. Polyclonal antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to the sequence of human PI3K p85, corresponding to human PI3K p110-gamma, surrounding Asp520 from human PI3K p110α, containing human PI3K p110β, and the amino-terminal sequence of human PI3K class III.

Background

Phosphoinositide 3-kinase (PI3K) catalyzes the production of phosphatidylinositol-3,4,5-triphosphate by phosphorylating phosphatidylinositol (PI), phosphatidylinositol-4-phosphate (PIP), and phosphatidylinositol-4,5-bisphosphate (PIP2). Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration, and cell survival (1). PTEN reverses this process, and research studies have shown that the PI3K signaling pathway is constitutively activated in human cancers that have loss of function of PTEN (2). PI3Ks are composed of a catalytic subunit (p110) and a regulatory subunit. Various isoforms of the catalytic subunit (p110α, p110β, p110γ, and p110δ) have been isolated, and the regulatory subunits that associate with p110α, p110β, and p110δ are p85α and p85β (3). In contrast, p110γ associates with a p101 regulatory subunit that is unrelated to p85. Furthermore, p110γ is activated by βγ subunits of heterotrimeric G proteins (4).

  1. Cantley, L.C. (2002) Science 296, 1655-7.
  2. Simpson, L. and Parsons, R. (2001) Exp Cell Res 264, 29-41.
  3. Neri, L.M. et al. (2002) Biochim Biophys Acta 1584, 73-80.
  4. Stoyanov, B. et al. (1995) Science 269, 690-3.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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