WB
H Mk
Endogenous
175
Rabbit IgG
#Q2NKX8
54821
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Monkey
Species predicted to react based on 100% sequence homology
The antigen sequence used to produce this antibody shares
100% sequence homology with the species listed here, but
reactivity has not been tested or confirmed to work by CST.
Use of this product with these species is not covered under
our
Product Performance Guarantee.
Hamster
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly610 of human PICH protein.
Background
PICH is a helicase of the SNF2 family of ATPases and is essential for proper chromosome segregation during mitosis (1). While PICH was originally proposed to participate in spindle assembly checkpoint signaling (1), that function was subsequently called into question (2). When phosphorylated at Thr1063 by CDK1, PICH binds the polo-box domain of the mitotic kinase PLK1 (1) and targets it to chromosome arms (3), where it appears to facilitate proper chromosome arm cohesion (4). PICH is also a substrate of PLK1 (1). Localized to the cytoplasm during interphase, PICH begins to accumulate at centromeres and kinetochores in prometaphase (4). As chromosomes begin to separate at the onset of anaphase, PICH associates with ultrafine threads between sister centromeres thought to be composed of entangled DNA (5), a natural consequence of DNA replication. PICH is proposed to cooperate with BLM, a RecQ-like helicase implicated in the genetic disorder Bloom’s Syndrome, to displace centromeric histones along these threads, thus enabling them to span large distances without breaking (6). This provides a temporal window for topoisomerase IIα-mediated disentanglement (7). Defects in PICH or BLM disrupt proper chromatid segregation and result in the formation of micronuclei (6).
- Baumann, C. et al. (2007) Cell 128, 101-14.
- Hübner, N.C. et al. (2010) Chromosoma 119, 149-65.
- Leng, M. et al. (2008) Cell Cycle 7, 1480-9.
- Kurasawa, Y. and Yu-Lee, L.Y. (2010) Mol Biol Cell 21, 1188-99.
- Wang, L.H. et al. (2008) Chromosoma 117, 123-35.
- Ke, Y. et al. (2011) EMBO J 30, 3309-21.
- Nitiss, J.L. (2009) Nat Rev Cancer 9, 327-37.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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