WB, IP
H M R Mk
Endogenous
75-95
Rabbit IgG
#Q8WUM9
6574
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly290 of human PiT1 protein.
Background
Phosphate transporter 1 (PiT1/SLC20A1) is a sodium dependent phosphate (Pi) transporter that imports Pi into cells. PiT1 was initially identified as a receptor for retroviruses (1,2). It is widely expressed in various tissues where it plays a critical role in maintaining cellular Pi homeostasis (3,4). Phosphate transporter 1 is important in cell proliferation and tumor cell growth independent of PiT1 phosphate transport function (5). Researchers have found that PiT1 is involved in TNF-α induced apoptosis (6). Moreover, phosphate uptake via PiT1 is crucial for vascular calcification (7) and overexpression of PiT1 leads to soft tissue calcification in Werner syndrome patients (8). Additional research indicates that increased PiT1 expression is seen in calcific aortic valve disease (CAVD) tissues, and that PiT1 enhances apoptosis and mineralization by modifying Akt1 levels (9).
- O'Hara, B. et al. (1990) Cell Growth Differ 1, 119-27.
- Miller, D.G. et al. (1994) Proc Natl Acad Sci U S A 91, 78-82.
- Kavanaugh, M.P. et al. (1994) Proc Natl Acad Sci U S A 91, 7071-5.
- Uckert, W. et al. (1998) Hum Gene Ther 9, 2619-27.
- Beck, L. et al. (2009) J Biol Chem 284, 31363-74.
- Salaün, C. et al. (2010) J Biol Chem 285, 34408-18.
- Li, X. et al. (2006) Circ Res 98, 905-12.
- Honjo, S. et al. (2008) Rejuvenation Res 11, 809-19.
- El Husseini, D. et al. (2013) PLoS One 8, e53393.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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