WB, IP
H M Mk
Endogenous
110
Rabbit IgG
#P21127
984
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Monkey
Species predicted to react based on 100% sequence homology
The antigen sequence used to produce this antibody shares
100% sequence homology with the species listed here, but
reactivity has not been tested or confirmed to work by CST.
Use of this product with these species is not covered under
our
Product Performance Guarantee.
Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Met734 of human PITSLRE/CDK11 protein.
Background
PITSLRE, alternatively known as cell division kinase 11 (CDK11), is the result of duplication of the CDK11 gene (1). CDK11A and CDK11B encode nearly identical serine/threonine protein kinases, PITSLREB and PITSLREA respectively, both belonging to the p34CDC2 family of protein kinases (2). Full-length PITSLRE/CDK11 (commonly referred to as CDK11p110) is expressed ubiquitously throughout the cell cycle whereas a smaller, alternate transcript (CDK11p58), the result of internal ribosomal entry, is expressed only during the G2/M transition where it promotes centrosome maturation and spindle formation (3-5). During induction of apoptosis by Fas or TNF, or anoikis, PITSLRE/CDK11 is cleaved by caspases to generate p110C, an approximately 46 kDa protein that contains the catalytically active kinase domain of PITSLRE/CDK11 that interacts with and inhibits p21-activated kinase (PAK1) activity (6-8). Full length PITSLRE/CDK11 (CDK11p110) appears to participate in pre-mRNA splicing events. This is demonstrated by the observation that CDK11p110 interacts with numerous splicing factors including RNPS1, 9G8/SRSF7 and cyclin L and that CDK11p110 can phosphorylate and inhibit the splicing activity of 9G8/SRSF7 (9-11).
- Gururajan, R. et al. (1998) Genome Res 8, 929-39.
- Xiang, J. et al. (1994) J Biol Chem 269, 15786-94.
- Cornelis, S. et al. (2000) Mol Cell 5, 597-605.
- Petretti, C. et al. (2006) EMBO Rep 7, 418-24.
- Wilker, E.W. et al. (2007) Nature 446, 329-32.
- Chen, S. et al. (2003) J Biol Chem 278, 20029-36.
- Lahti, J.M. et al. (1995) Mol Cell Biol 15, 1-11.
- Ariza, M.E. et al. (1999) J Biol Chem 274, 28505-13.
- Hu, D. et al. (2003) J Biol Chem 278, 8623-9.
- Loyer, P. et al. (1998) J Cell Sci 111 ( Pt 11), 1495-506.
- Dickinson, L.A. et al. (2002) J Biol Chem 277, 25465-73.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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