| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| Phospho-PLCγ2 (Tyr1217) Antibody | 3871 | 20 µl | 150 kDa | Rabbit |
| Phospho-PLCγ2 (Tyr759) (E9E9Y) Rabbit mAb | 50535 | 20 µl | 150 kDa | Rabbit IgG |
| PLCγ2 (E5U4T) Rabbit mAb | 55512 | 20 µl | 150 kDa | Rabbit IgG |
| Phospho-PLCγ1 (Tyr783) (D6M9S) Rabbit mAb | 14008 | 20 µl | 155 kDa | Rabbit IgG |
| PLCγ1 (D9H10) XP® Rabbit mAb | 5690 | 20 µl | 150 kDa | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat |
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Description
PLCγ Antibody Sampler Kit provides an economical means of analyzing phospho and total PLCγ levels. PLCγ Antibody Sampler Kit contains enough primary and secondary antibodies to perform two western blot experiments with each antibody.
Storage
Background
Phosphoinositide-specific phospholipase C (PLC) plays a significant role in transmembrane signaling. In response to extracellular stimuli, such as hormones, growth factors, and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to generate two secondary messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG) (1). At least four families of PLCs have been identified: PLCβ, PLCγ, PLCδ, and PLCε. Phosphorylation is one of the key mechanisms that regulate the activity of PLC. PLCγ is activated by both receptor and non-receptor tyrosine kinases (2). PLCγ forms a complex with EGF and PDGF receptors, which leads to the phosphorylation of PLCγ at Tyr771, 783, and 1248 (3). Phosphorylation by Syk at Tyr783 activates the enzymatic activity of PLCγ1 (4). PLCγ2 is engaged in antigen-dependent signaling in B cells and collagen-dependent signaling in platelets. Phosphorylation by Btk or Lck at Tyr753, 759, 1197, and 1217 is correlated with PLCγ2 activity (5,6).
Background References
Trademarks and Patents
Limited Uses
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