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3860
PLCγ Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

PLCγ Antibody Sampler Kit #3860

Citations (1)
Simple Western™ analysis of lysates (0.1 mg/mL) from serum-starved A431 cells treated with hEGF (100 ng/mL, 5 min.) using Phospho-PLCγ1 (Tyr783) (D6M9S) Rabbit mAb #14008. The virtual lane view (left) shows a single target band (as indicated) at 1:50 and 1:250 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:50 (blue line) and 1:250 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.
Immunoprecipitation of PLCγ1 protein from HeLa cell extracts. Lane 1 is 10% input, lane 2 is PLCγ1 (D9H10) XP® Rabbit mAb, and lane 3 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900. Western blot analysis was performed using PLCγ1 (D9H10) XP® Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody.
Simple Western™ analysis of lysates (1mg/mL) from 3T3 cells using PLCγ1 (D9H10) XP® Rabbit mAb #5690. The virtual lane view (left) shows the target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the12-230 kDa separation module.
Western blot analysis of extracts from NIH/3T3 cells, untreated (-) or stimulated with hPDGF-BB #8912 (5 min; +), and from A-431 cells, untreated (-) or stimulated with hEGF #8916 (5 min; +), using Phospho-PLCγ1 (Tyr783) (D6M9S) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western blot analysis of extracts from Ramos cells, untreated or treated with anti-human IgM, using Phospho-PLCgamma2 (Tyr1217) Antibody (upper) or PLCgamma2 Antibody #3872 (lower).
Western blot analysis of extracts from Daudi cells, untreated (-) or treated with anti-human IgM (12 μg/ml, 10 min; +), using Phospho-PLCγ2 (Tyr759) (E9E9Y) Rabbit mAb (upper), PLCγ2 (E5U4T) Rabbit mAb #55512 (middle), or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Western blot analysis of extracts from various cell lines using PLCγ2 (E5U4T) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western blot analysis of extracts from various cell lines using PLCγ1 (D9H10) XP® Rabbit mAb.
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
Immunoprecipitation of phospho-PLCγ1 (Tyr783) from A-431 cell extracts stimulated with hEGF #8916 using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Phospho-PLCγ1 (Tyr783) (D6M9S) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Phospho-PLCγ1 (Tyr783) (D6M9S) Rabbit mAb.
Immunoprecipitation of PLCγ2 from HCT 116 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is PLCγ2 (E5U4T) Rabbit mAb. Western blot analysis was performed using PLCγ2 (E5U4T) Rabbit mAb.
Western blot analysis of extracts from HeLa cells, either mock-transfected or transfected for 48 hours with SignalSilence PLCγ1 siRNA I #6293 or siRNA II #6254, using PLCγ1 (D9H10) XP® Rabbit mAb.
Confocal immunofluorescent analysis of HCT 116 cells (left, positive) and 22Rv1 cells (right, negative) using PLCγ2 (E5U4T) Rabbit mAb (green). Actin filaments were labeled with DyLight 554 Phalloidin #13054 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using PLCγ1 (D9H10) XP® Rabbit mAb.
Flow cytometric analysis of NIH/3T3 cells, untreated (blue) or treated with mPDGFbb (200ng/mL, 15 min; green) using Phospho-PLCγ1 (Tyr783) (D6M9S) Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Immunohistochemical analysis of paraffin-embedded mouse pancreas using PLCγ1 (D9H10) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse spleen using PLCγ1 (D9H10) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse testis using PLCγ1 (D9H10) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human lung carcioma using PLCγ1 (D9H10) XP® Rabbit mAb.
To Purchase # 3860
Cat. # Size Qty. Price
3860T
1 Kit  (5 x 20 microliters)

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-PLCγ2 (Tyr1217) Antibody 3871 20 µl
  • WB
H M 150 Rabbit 
Phospho-PLCγ2 (Tyr759) (E9E9Y) Rabbit mAb 50535 20 µl
  • WB
H 150 Rabbit IgG
PLCγ2 (E5U4T) Rabbit mAb 55512 20 µl
  • WB
  • IP
  • IF
H 150 Rabbit IgG
Phospho-PLCγ1 (Tyr783) (D6M9S) Rabbit mAb 14008 20 µl
  • WB
  • IP
  • F
H M 155 Rabbit IgG
PLCγ1 (D9H10) XP® Rabbit mAb 5690 20 µl
  • WB
  • IP
  • IHC
H M R Mk 150 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

Product Description

PLCγ Antibody Sampler Kit provides an economical means of analyzing phospho and total PLCγ levels. PLCγ Antibody Sampler Kit contains enough primary and secondary antibodies to perform two western blot experiments with each antibody.

Specificity / Sensitivity

Each antibody in the PLCγ Antibody Sampler Kit detects endogenous levels of its target protein. The antibodies do not cross react with other PLCs.

Source / Purification

Monoclonal phospho-specific antibodies are produced by immunizing animals with a synthetic phospho-peptide corresponding to residues surrounding Leu1220 of human PLCγ1 protein, Tyr759 of human PLCγ2 protein, or Tyr783 of PLCγ1. Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human PLCγ2 protein.

Polyclonal antibodies are produced by immunizing animals with a synthetic phospho-peptide corresponding to residues surrounding Tyr1217 of human PLCgamma2. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Phosphoinositide-specific phospholipase C (PLC) plays a significant role in transmembrane signaling. In response to extracellular stimuli, such as hormones, growth factors, and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to generate two secondary messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG) (1). At least four families of PLCs have been identified: PLCβ, PLCγ, PLCδ, and PLCε. Phosphorylation is one of the key mechanisms that regulate the activity of PLC. PLCγ is activated by both receptor and non-receptor tyrosine kinases (2). PLCγ forms a complex with EGF and PDGF receptors, which leads to the phosphorylation of PLCγ at Tyr771, 783, and 1248 (3). Phosphorylation by Syk at Tyr783 activates the enzymatic activity of PLCγ1 (4). PLCγ2 is engaged in antigen-dependent signaling in B cells and collagen-dependent signaling in platelets. Phosphorylation by Btk or Lck at Tyr753, 759, 1197, and 1217 is correlated with PLCγ2 activity (5,6).

Pathways

Explore pathways related to this product.

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.
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