Immunohistochemical analysis of paraffin-embedded human thyroid papillary carcinoma using PLD2 (E1Y9L) Rabbit mAb (IHC Specific).Learn more about how we get our images
Immunohistochemical analysis of paraffin-embedded human lung non-small cell carcinoma using PLD2 (E1Y9L) Rabbit mAb (IHC Specific).Learn more about how we get our images
Immunohistochemical analysis of paraffin-embedded human ovarian serous carcinoma using PLD2 (E1Y9L) Rabbit mAb (IHC Specific) in the presence of control peptide (left) or antigen specific peptide (right).Learn more about how we get our images
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
NOTE: Do not allow slides to dry at any time during this procedure.
For Citrate: Heat slides in a microwave submersed in 1X citrate unmasking solution until boiling is initiated; follow with 10 min at a sub-boiling temperature (95°-98°C). Cool slides on bench top for 30 min.
posted February 2010
revised March 2016
Protocol Id: 283
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
PLD2 (E1Y9L) Rabbit mAb recognizes endogenous levels of total PLD2 protein.
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala880 of human PLD2 protein.
Phosphatidylcholine-specific phospholipase D (PLD) hydrolyzes phosphatidylcholine (PC) to produce choline and phosphatidic acid (PA). PA is the precursor of the second messenger, diacylglycerol (DAG). Two isoforms of PLD (PLD1 and PLD2) have been identified so far. Both are regulated by protein kinases, small GTPases and Ca2+ (1). The PLD2 isoform is highly expressed in many cancers, such as colorectal and breast cancers (2,3). PLD2 also acts as a guanine nucleotide exchange factor for the small GTPase Rac2 independent of its phospholipase activity (4).
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|13891S||100 µl (250 sections)||$ 255.0|