WB
H M
Endogenous
80
Rabbit IgG
#Q9H4B4
1263
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse
Species predicted to react based on 100% sequence homology
The antigen sequence used to produce this antibody shares
100% sequence homology with the species listed here, but
reactivity has not been tested or confirmed to work by CST.
Use of this product with these species is not covered under
our
Product Performance Guarantee.
Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Cys625 of human PLK3 protein.
Background
At least 4 distinct polo-like kinases exist in mammalian cells: PLK1, PLK2, PLK3 and PLK4/SAK (1). Like the other PLK family members, PLK3 contains an amino-terminal catalytic domain and a conserved carboxy-terminal domain termed the Polo box (2). PLK3, also called proliferation-related kinase (Prk) (3), was originally described as a fibroblast growth factor (FGF)-inducible kinase (Fnk) and identified as an immediate-early response gene responsive to FGF-1 and other mitogens (4). PLK3 is a cytokine-inducible serine/threonine kinase whose protein expression is cell cycle regulated. Though its expression is found primarily in G1 phase of the cell cycle, PLK3 is detected in G0 and in late telophase prior to cytokinesis (5). Like the other PLK family members, PLK3 functions mainly as a regulator of the cell cycle. Specifically, PLK3 is required for entry into S phase and is a critical regulator of G1 events, as indicated by RNAi-induced PLK3-depleted cells (2). PLK3 is also involved in the regulation of DNA damage response via phosphorylation of p53 on Ser20 (6). PLK3 may act as a tumor suppressor as Plk3-deficient mice develop spontaneous tumors in various organs (7). Unlike PLK1, PLK3 expression is down regulated in cancers including lung (3), head and neck (8), and colon (9).
- Nigg, E.A. (1998) <cite>Curr Opin Cell Biol</cite> 10, 776-83.
- Zimmerman, W.C. and Erikson, R.L. (2007) <cite>Proc Natl Acad Sci USA</cite> 104, 1847-52.
- Li, B. et al. (1996) <cite>J Biol Chem</cite> 271, 19402-8.
- Donohue, P.J. et al. (1995) <cite>J Biol Chem</cite> 270, 10351-7.
- Zimmerman, W.C. and Erikson, R.L. (2007) <cite>Cell Cycle</cite> 6, 1314-8.
- Xie, S. et al. (2001) <cite>J Biol Chem</cite> 276, 43305-12.
- Yang, Y. et al. (2008) <cite>Cancer Res</cite> 68, 4077-85.
- Dai, W. et al. (2000) <cite>Genes Chromosomes Cancer</cite> 27, 332-6.
- Dai, W. et al. (2002) <cite>Int J Oncol</cite> 20, 121-6.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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