Order 3, Get Your 4th Free!* | Learn More >>
9093
StemLight™ Pluripotency Transcription Factor Antibody Kit
Primary Antibodies
Antibody Sampler Kit

StemLight™ Pluripotency Transcription Factor Antibody Kit #9093

Reviews ()
Citations (0)
StemLight™ Pluripotency Transcription Factor Antibody Kit: Image 1

Flow cytometric analysis of HeLa cells (blue) and NTERA-2 cells (green) using Nanog (D73G4) XP® Rabbit mAb.

StemLight™ Pluripotency Transcription Factor Antibody Kit: Image 2

Flow cytometric analysis of HeLa cells (blue) and NCCIT cells (green) using Oct-4A (C30A3) Rabbit mAb.

StemLight™ Pluripotency Transcription Factor Antibody Kit: Image 3

Flow cytometric analysis of HeLa cells (blue) and NTERA2 cells (green) using Sox2 (D6D9) XP® Rabbit mAb.

StemLight™ Pluripotency Transcription Factor Antibody Kit: Image 4

Confocal immunofluorescent analysis of NTERA-2 cells (left) and HeLa cells (right) using Nanog (D73G4) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).

StemLight™ Pluripotency Transcription Factor Antibody Kit: Image 5

Confocal immunofluorescent analysis of NTERA2 (left) and mouse embryonic stem (mES) cells growing on mouse embryonic fibroblast (MEF) feeder cells (right) using Oct-4A (C30A3) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).

StemLight™ Pluripotency Transcription Factor Antibody Kit: Image 6

Confocal immunofluorescent analysis of NTERA2 (left) and HeLa (right) cells using Sox2 (D6D9) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).

StemLight™ Pluripotency Transcription Factor Antibody Kit: Image 7

Immunohistochemical analysis of paraffin-embedded human seminoma using Nanog (D73G4) XP® Rabbit mAb.

StemLight™ Pluripotency Transcription Factor Antibody Kit: Image 8

Western blot analysis of extracts from NTERA2 and mouse embryonic stem cells (mESCs) using Oct-4A (C30A3) Rabbit mAb.

StemLight™ Pluripotency Transcription Factor Antibody Kit: Image 9

Western blot analysis of extracts from NCCIT, NTERA-2 and iPS cells using Nanog (D73G4) XP® Rabbit mAb.

StemLight™ Pluripotency Transcription Factor Antibody Kit: Image 10

Western blot analysis of extracts from NTERA2 and NCCIT cells using Sox2 (D6D9) XP® Rabbit mAb.

To Purchase # 9093T
Product # Size Price
9093T
1 Kit  (3 x 20 µl) $ 261

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Nanog (D73G4) XP® Rabbit mAb 4903 20 µl
  • WB
  • IHC
  • IF
  • F
H 42 Rabbit IgG
Oct-4A (C30A3) Rabbit mAb 2840 20 µl
  • WB
  • IF
  • F
H M 45 Rabbit IgG
Sox2 (D6D9) XP® Rabbit mAb 3579 20 µl
  • WB
  • IF
  • F
H 35 Rabbit 

Product Description

StemLight™ Pluripotency Transcription Factor Antibody Kit contains a panel of antibodies for the detection of Oct-4, Nanog, and Sox2, key components of the core pluripotency transcription network in embryonic stem (ES) and induced pluripotent stem (iPS) cells. The kit can be used to track the pluripotent potential of human ES or iPS cells. The loss of these markers indicates a loss of pluripotency or differentiation of the culture. The kit components are pre-optimized for parallel use in immunofluorescent analysis at a standard dilution, but components are also validated for use in other applications - please refer to individual datasheet information for application specific recommendations. Enough reagents are provided for 160 immunofluorescent assays based on a working volume of 100 μl.

Specificity / Sensitivity

Nanog (D73G4) XP® Rabbit mAb detects endogenous levels of total Nanog protein. Oct-4A (C30A3) Rabbit mAb detects endogenous levels of total Oct-4A protein. Sox2 (D6D9) XP® Rabbit mAb detects endogenous levels of total Sox2 protein.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human Nanog protein, recombinant protein corresponding to the amino terminus of human Oct-4A, and a synthetic peptide corresponding to residues surrounding Gly179 of human Sox2.

Background

Pluripotency is the ability of a cell to differentiate into cell types of the three germ layers, the endoderm, ectoderm and mesoderm. It is a property shared by embryonic stem cells, embryonic carcinoma, and induced pluripotent cells.

Oct-4, Sox2, and Nanog are key transcriptional regulators that are highly expressed in pluripotent cells (1). Together they form a transcriptional network that maintains cells in a pluripotent state (2,3). Over-expression of Oct-4 and Sox2, along with KLF4 and c-Myc can induce pluripotency in both mouse and human somatic cells, highlighting their roles as key regulators of the transcriptional network necessary for renewal and pluripotency (4-5). It has also been demonstrated that overexpression of Oct-4, Sox2, Nanog, and Lin28 can induce pluripotency in human somatic cells (6). Upon differentiation of pluripotent cultures, expression of Oct-4, Nanog, and Sox2 is downregulated.

  1. Looijenga, L.H. et al. (2003) Cancer Res 63, 2244-50.
  2. Pesce, M. and Schöler, H.R. (2001) Stem Cells 19, 271-8.
  3. Pan, G. and Thomson, J.A. (2007) Cell Res 17, 42-9.
  4. Takahashi, K. and Yamanaka, S. (2006) Cell 126, 663-76.
  5. Okita, K. et al. (2007) Nature 448, 313-7.
  6. Yu, J. et al. (2007) Science 318, 1917-20.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
StemLight™ is a trademark of Cell Signaling Technology, Inc.
DRAQ5 is a registered trademark of Biostatus Limited.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.